Characterization of Mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazil

• Autor(es): Rabahi, Marcelo Fouad ; Concei??o, Emilyn Costa ; Paiva, Lu?sa Oliveira de ; Souto, Marcos Vin?cius Muniz Lemes ; Sisco, Maria Carolina ; Waard, Jacobus de ; Caldas, Paulo Cesar de Souza ; Fandinho, Fatima ; Ramos, Jesus Pais ; Carvalho, Luciana Distasio de ; Campos, Carlos Eduardo Dias ; Lima, Karla Val?ria Batista ; Silva, Sandro Patroca da ; Sharma, Abhinav ; Robledo, Jaime ; Paez, Uriel Alonso Hurtado ; Duarte, Rafael Silva ; Silva, Marlei Gomes da ; Gomes, Lia Lima ; Vasconcellos, Sidra Ezidio Gon?alves ; Uwezeye, Cecile ; Jong, Bouke C. de ; Junqueira-Kipnis, Ana Paula ; Suffys, Philip Noel
• Data: 2020
• Origem: Oasisbr
• Assunto(s): Tuberculose Pulmonar / patologia; Mycobacterium tuberculosis / patogenicidade; Mycobacterium tuberculosis var. africanum; Genoma Bacteriano; Relatos de Casos
• Tipo de Documento: Artigo científico
• Idioma: Inglês

Descrição

Universidade Federal de Goi?s. Hospital das Cl?nicas. Goi?nia, GO, Brazil.

Funda??o Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Programa de P?s-gradua??o em Pesquisa Cl?nica e Doen?as Infecciosas. Rio de Janeiro, RJ, Brazil / Funda??o Oswaldo Cruz. Instituto Oswaldo Cruz. Laborat?rio de Biologia Molecular Aplicada em Micobacterias. Rio de Janeiro, RJ, Brazil.

Universidade Federal de Goi?s. Hospital das Cl?nicas. Goi?nia, GO, Brazil.

Universidade Federal de Goi?s. Hospital das Cl?nicas. Goi?nia, GO, Brazil.

Funda??o Oswaldo Cruz. Instituto Oswaldo Cruz. Laborat?rio de Biologia Molecular Aplicada em Micobacterias. Rio de Janeiro, RJ, Brazil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de G?es. Rio de Janeiro, RJ, Brazil.

Instituto de Biomedicina Dr. Jacinto Convit. Instituto de Biomedicina. San Jose, Caracas, Venezuela / Universidad de Las Am?ricas. Facultad de Ciencias de La Salud. One Health Research Group. Quito, Ecuador.

Funda??o Oswaldo Cruz. Centro de Refer?ncia Professor H?lio Fraga. Rio de Janeiro, RJ, Brazil.

Funda??o Oswaldo Cruz. Centro de Refer?ncia Professor H?lio Fraga. Rio de Janeiro, RJ, Brazil.

Funda??o Oswaldo Cruz. Centro de Refer?ncia Professor H?lio Fraga. Rio de Janeiro, RJ, Brazil.

Funda??o Oswaldo Cruz. Centro de Refer?ncia Professor H?lio Fraga. Rio de Janeiro, RJ, Brazil.

Funda??o Oswaldo Cruz. Centro de Refer?ncia Professor H?lio Fraga. Rio de Janeiro, RJ, Brazil.

Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.

Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.

International Institute of InformationTechnology - Bangalore. Department of Data Science. Bangalore, India.

Corporaci?n para Investigaciones Biol?gicas. Medell?n, Colombia.

Corporaci?n para Investigaciones Biol?gicas. Medell?n, Colombia.

Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de G?es. Rio de Janeiro, RJ, Brazil.

Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de G?es. Rio de Janeiro, RJ, Brazil.

Funda??o Oswaldo Cruz. Instituto Oswaldo Cruz. Laborat?rio de Biologia Molecular Aplicada em Micobacterias. Rio de Janeiro, RJ, Brazil.

Funda??o Oswaldo Cruz. Instituto Oswaldo Cruz. Laborat?rio de Biologia Molecular Aplicada em Micobacterias. Rio de Janeiro, RJ, Brazil.

Institute of Tropical Medicine. Mycobacteriology Unit. Antwerp, Belgium.

Institute of Tropical Medicine. Mycobacteriology Unit. Antwerp, Belgium.

Universidade Federal de Goi?s. Instituto de Patologia Tropical e Sa?de P?blica. Goi?nia, GO, Brazil.

Funda??o Oswaldo Cruz. Instituto Oswaldo Cruz. Laborat?rio de Biologia Molecular Aplicada em Micobacterias. Rio de Janeiro, RJ, Brazil.

Human tuberculosis (TB) is caused by members of the Mycobacterium tuberculosis complex (MTBC), including Mycobacterium tuberculosis var. tuberculosis (MTB) and Mycobacterium tuberculosis var. africanum (MAF). While MTB is isolated worldwide, MAF is almost completely restricted to the African continent, and despite the historical proximity between Brazil and Africa during the slave trade, no case of TB being caused by MAF has been reported in Brazil to date. We hereby describe the first case of TB caused by MAF in Brazil comparing its genome against the published ones. A female patient who had never visited Africa presented with clinical symptoms typical of pulmonary TB. Based on 16S rRNA gene sequencing, the cultured isolate was identified as belonging to MTBC and partial sequence of the hsp65 gene was identical to that of MAF. This was confirmed by genotyping based on detection of Single Nucleotide Polymorphism (SNP), Region of Difference (RD) and spoligotyping. The isolate presented the Shared International Typing (SIT) 181. In the whole-genome comparison against MAF genomes available on published EMBL-EBI European Nucleotide Archive (ENA), the Brazilian genome (MAFBRA00707) was identified as belonging to Lineage 6 and clustered with isolates from The Gambia. This is the first report of the isolation of MAF from a patient from Brazil, without evidence of having any contact with an African index case