Autor(es):
Ferreira, Raquel da Silva ; Cruz, Lucin?ia Claudia de Toni Aquino da ; Souza, Vilma Jucineide ; Neves, Nilvanei Aparecido da Silva ; Souza, Victor Costa de ; Franco Filho, Luciano Chaves ; Lemos, Poliana da Silva ; Lima, Clayton Pereira Silva de ; Naveca, Felipe Gomes ; Atanaka, Marina ; Nunes, Marcio Roberto Teixeira ; Slhessarenko, Renata Dezengrini
Data: 2020
Origem: Oasisbr
Assunto(s): Alphavirus / isolamento & purifica??o; Insetos / virologia; Culicidae / virologia; Arbovirus / patogenicidade; Mato Grosso (MT); Flavivirus / isolamento & purifica??o
Descrição
Universidade Federal de Mato Grosso. Programa de P?s-Gradua??o em Ci?ncias da Sa?de. Laborat?rio de Virologia. Cuiab?, MT, Brazil.
Universidade Federal de Mato Grosso. Instituto de Sa?de Coletiva. Programa de P?s-Gradua??o em Ci?ncias da Sa?de. Cuiab?, MT, Brazil.
Pal?cio Paiagu?s. Centro Pol?tico Administrativo. Secretaria Estadual de Sa?de. Cuiab?, MT, Brazil.
Universidade Federal de Mato Grosso. Programa de P?s-Gradua??o em Ci?ncias da Sa?de. Laborat?rio de Virologia. Cuiab?, MT, Brazil.
Funda??o Oswaldo Cruz. Instituto Le?nidas e Maria Deane. Manaus, AM, Brazil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Funda??o Oswaldo Cruz. Instituto Le?nidas e Maria Deane. Manaus, AM, Brazil.
Universidade Federal de Mato Grosso. Instituto de Sa?de Coletiva. Programa de P?s-Gradua??o em Ci?ncias da Sa?de. Cuiab?, MT, Brazil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Universidade Federal de Mato Grosso. Programa de P?s-Gradua??o em Ci?ncias da Sa?de. Laborat?rio de Virologia. Cuiab?, MT, Brazil.
Viruses were identified from male anthropophilic mosquitoes from Mato Grosso (MT) State, Midwest Brazil from February 2017 to January 2018. Mosquitoes tested included Aedes (Stegomyia) aegypti (1139 males; 84 pools), Culex quinquefasciatus (9426 males; 179 pools), Culex sp. (3 males; 3 pools) and Psorophora albigenu (1 male; 1 pool) collected from four cities of MT. Pools were subjected to viral RNA extraction followed by RT-PCRs specific for ten flaviviruses, five alphaviruses and Simbu serogroup of orthobunyaviruses. Positive pools were passaged three times in VERO cells (alphavirus and orthobunyavirus) or C6/36 cells (flavivirus), with isolates confirmed through RT-PCR and nucleotide sequencing. We detected pools positive for Ilh?us (1 pool), dengue serotype 4 (1), Mayaro (12), equine encephalitis virus (1) yellow fever (1), Oropouche (2), Zika (4) and chikungunya (12) viruses. High throughput sequencing of arbovirus positive pools identified 35 insect-specific viruses (ISVs) from the families Circoviridae (2), Parvoviridae (2), Totiviridae (1), Flaviviridae (1), Iflaviridae (2), Mesoniviridae (4), Nodaviridae (2), Luteoviridae (1), Phasmaviridae (1) Phenuiviridae (2), Rhabdoviridae (2), Orthomyxoviridae (1), Xinmoviridae (1), and unclassified Bunyavirales (1), unclassified Picornavirales (3), unclassified Riboviria (4) and taxon Negevirus (5). From these, five novel viruses were tentatively named Mojica circovirus, Kuia iflavirus, Muxirum negevirus, Lambada picorna-like virus and Tacuru picorna-like virus. Our findings underscore the diversity and wide geographical distribution of ISVs and arboviruses infecting male culicids.
