Autor(es): Silva, Aniel de Sarom Negr?o ; Lima, Clayton Pereira Silva de ; Barata, Rafael Ribeiro ; Silva, Pedro Victor Reis da ; Monteiro, Patricia Danin Jord?o ; Lamar?o, Let?cia ; Burbano, Rommel M?rio Rodr?gues ; Nunes, M?rcio Roberto Teixeira ; Lima, Danielle Lima de
Data: 2020
Origem: Oasisbr
Assunto(s): Sangue / virologia; HIV / gen?tica; Infec??es por V?rus de RNA / sangue; Coinfec??o / sangue; Coinfec??o / virologia; Carga Viral; Preval?ncia; Doadores de Sangue
HEMOPA foundation, Evandro Chagas Institute, Foundation for Scientific and Technological Development in Health (FIOTEC; Project PRES-012-FIO-16) and funded in part by the research productivity project CNPq (302584/2015?3)
Par? State University. Center for Life Science and Health. Bel?m, PA, Brazil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Par? State University. Center for Life Science and Health. Bel?m, PA, Brazil.
Foundation Center for Hemotherapy and Hematology of Par?. Bel?m, PA, Brazil.
Foundation Center for Hemotherapy and Hematology of Par?. Bel?m, PA, Brazil.
Ophir Loyola Hospital. Bel?m, PA, Brazil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Par? State University. Center for Life Science and Health. Bel?m, PA, Brazil.
Human pegivirus (HPgV)?formerly known as GBV-C?is a member of the Flaviviridae family and belongs to the species Pegivirus C. It is a non-pathogenic virus and is transmitted among humans mainly through the exposure to contaminated blood and is often associated with human immunodeficiency virus (HIV) infection, among other viruses. This study aimed to determine the prevalence of HPgV viremia, its association with HIV and clinical epidemiological factors, as well as the full-length sequencing and genome characterization of HPgV recovered from blood donors of the HEMOPA Foundation in Bel?m-PA-Brazil. Methods: Plasma samples were obtained from 459 donors, tested for the presence of HPgV RNA by the RT-qPCR. From these, a total of 26 RT-qPCR positive samples were submitted to the NGS sequencing approach in order to obtain the full genome. Genome characterization and phylogenetic analysis were conducted. Results: The prevalence of HPgV was 12.42%. We observed the highest prevalences among donors aged between 18 and 30 years old (16.5%), with brown skin color (13.2%) and men (15.8%). The newly diagnosed HIV-1 prevalence was 26.67%. The HPgV genotype 2 (2a and 2b) was identified. No data on viral load value was found to corroborate the protective effect of HPgV on HIV evolution. Conclusions: This study provided information regarding the HPgV infection among blood donors from HEMOPA Foundation. Furthermore, we genetically characterized the HPgV circulating strains and described by the first time nearly complete genomes of genotype 2 in Brazilian Amazon.
