Detection and molecular characterization of rotavirus and picobirnavirus in wild avians from Amazon Forest

• Autor(es): Duarte J?nior, Jos? Wandilson Barboza ; Chagas, Elaine Hellen Nunes ; Serra, Ana Carolina Silva ; Souto, Lizandra Caroline dos santos ; Penha J?nior, Edvaldo Tavares da ; Bandeira, Renato da Silva ; Guimar?es, Ricardo Jos? de Paula Souza e ; Oliveira, Hanna Gabriela da Silva ; Sousa, Thaymes Kiara Santos ; Lopes, Cinthia T?vora de Albuquerque ; Domingues, Sheyla Farhayldes Souza ; Malik, Yashpal Singh ; Salvarani, Felipe Masiero ; Mascarenhas, Joana D?Arc Pereira
• Data: 2020
• Origem: Oasisbr
• Assunto(s): Aves / virologia; Rotavirus / isolamento & purifica??o; Picobirnavirus / isolamento & purifica??o; Hospitais Veterin?rios
• Tipo de Documento: Artigo científico
• Idioma: Inglês

Descrição

Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.

Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.

Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.

Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.

Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.

Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.

Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Laborat?rio de Geoprocessamento. Ananindeua, PA, Brasil.

University of the State of Par?. Institute of Veterinary Medicine. Castanhal, PA, Brazil.

University of the State of Par?. Institute of Veterinary Medicine. Castanhal, PA, Brazil.

University of the State of Par?. Institute of Veterinary Medicine. Castanhal, PA, Brazil.

University of the State of Par?. Institute of Veterinary Medicine. Castanhal, PA, Brazil.

Indian Veterinary Research Institute. India

University of the State of Par?. Institute of Veterinary Medicine. Castanhal, PA, Brazil.

Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.

The present study reports the detection and molecular characterization of rotavirus A (RVA), rotavirus D (RVD), rotavirus F (RVF), rotavirus G (RVG) and picobirnavirus (PBV) in fecal specimens of wild and exotic birds (n = 23) from different cities of Par? state, which were hospitalized at Veterinary Hospital of the Federal University of Par?, Brazil, between January 2018 to June 2019. The animals exhibited different clinical signs, such as diarrhea, malnutrition, dehydration and fractures. The results showed 39.1% (9/23) of positivity for RVA by RT-qPCR. Among these, one sample (1/9) for the NSP3 gene of T2 genotype was characterized. About 88.9% (8/9) for the VP7 gene belonging to G1, equine-like G3 and G6 genotypes, and 55.5% (5/9) for the VP4 gene of P[2] genotype were obtained. In the current study, approximately 4.5% of the samples (1/23) revealed coinfection for the RVA, RVD and RVF groups. Furthermore, picobirnavirus (PBV) was detected in 1 of the 23 samples tested and was classified in the Genogroup I. The findings represent the first report of the circulation of RVA, RVD, RVF, RVG and PBV genotypes in wild birds in Brazil and suggest the possible interspecies transmission of RVs and PBVs.