Autor(es): Gon?alves, Lucas Pantoja ; Santos, Thiago Vasconcelos dos ; Campos, Marliane Batista ; Lima, Luciana Vieira do R?go ; Ishikawa, Edna Aoba Yassui ; Silveira, Fernando Tobias ; Ramos, Patr?cia Karla Santos
Data: 2021
Origem: Oasisbr
Assunto(s): Leishmaniose Cut?nea / patologia; Leishmaniose Cut?nea / etiologia; Rea??o em Cadeia da Polimerase / m?todos
This research was financially supported by the Instituto Evandro Chagas (Brazilian Ministry of Health) and by the N?cleo de Medicina Tropical (Universidade Federal do Par?, Brasil). Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (editals no.1/2017- PIBIC/IEC/CNPq 2017-2018 and no.1/2016-PIBIC/IEC/CNPq 2016-2017)
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Universidade Federal do Par?. N?cleo de Medicina Tropical. Bel?m, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Introduction: In the Bel?m Metropolitan Region (BMR), Par? State, Brazil, American cutaneous leishmaniasis (ACL) is endemic; however, very little is known regarding its causative agents. Therefore, we used our standard diagnostic approach combined with an RNA polymerase II largest subunit (RNAPOIILS)-polymerase chain reaction (PCR) followed by analysis of restriction fragment length polymorphism (PCR-RFLP) to identify Leishmania spp. ACL agents in this region. Methods: Thirty-two Leishmania spp. isolates from patients with ACL in the BMR during 1995-2018 were analyzed. Leishmania spp. DNA samples were amplified using the primers RPOR2/RPOF2, and the 615-bp PCR products were subjected to enzymatic digestion using TspRI and HgaI endonucleases. Results: ACL etiological agents in the BMR comprised Leishmania (Viannia) lindenbergi (43.7%) followed by Leishmania (Viannia) lainsoni (34.4%), Leishmania (Leishmania) amazonensis (12.5%), and Leishmania (Viannia) braziliensis (9.4%). Conclusions: To our knowledge, the results of the study revealed for the first time that L. (V.) lindenbergi and L. (V.) lainsoni are the main ACL agents in BMR.
