Autor(es): Dias Junior, Elcio Malcher ; Dias, Dayane dos Reis Costa ; Rodrigues, Ana Paula Drummond ; Dias, Carmen Gilda Barroso Tavares ; Bastos, Gilmara de Nazareth Tavares ; Oliveira, Johnatt Allan Rocha de ; Maciel Filho, Rubens ; Passos, Marcele Fonseca
Data: 2021
Origem: Oasisbr
Assunto(s): Cartilagem / ultraestrutura; Materiais Biocompat?veis; Medicina Regenerativa; Poli-Hidroxietil Metacrilato; Engenharia Tecidual
Federal University of Par?. Institute of Biological Sciences. Bel?m, PA, Brazil.
Federal University of Par?. Institute of Biological Sciences. Bel?m, PA, Brazil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Laborat?rio de Microscopia. Ananindeua, PA, Brasil.
Federal University of Par?. Department of Mechanical Engineering. Bel?m, PA, Brazil.
Federal University of Par?. Institute of Biological Sciences. Bel?m, PA, Brazil.
Federal University of Par?. Department of Nutrition. Bel?m, PA, Brazil.
Campinas State University. National Institute of Science and Technology in Biofabrication. Chemical Engineering Department. Campinas, SP, Brazil.
Federal University of Par?. Institute of Biological Sciences. Bel?m, PA, Brazil / Campinas State University. National Institute of Science and Technology in Biofabrication. Chemical Engineering Department. Campinas, SP, Brazil.
Despite the many advances in the field of regenerative medicine, the use of biomaterials to repair the cartilaginous tissue's traumatic injuries is still a challenge. Based on that, this research focuses on the semi-interpenetrating polymeric scaffolds (SIPNs) based PLLA and PHEMA. The material was produced in the solvent absence as a strategy to outline the critical limitations of hydrophilicity and the lack of biological interaction of biomaterials with the surrounding tissues. To this end, the thermal properties and chemical structure were characterized using thermogravimetric analysis and spectroscopy in the Fourier transform infrared. Besides, we assessed porosity and tissue interactions by scanning electron microscopy, and the influence of this in the swelling capacity tests. In vitro and in vivo assays and histological analysis were developed to assess the biocompatibility of SIPNs scaffolds. The results showed good thermal stability, porosity in the range of 53.73 to 94.34??m, absence of toxicity, and excellent in vitro cell adhesion and growth. The biomaterial also promoted a great template for cell migration and spreading in vivo, with high cartilaginous tissue remodeling. SIPNs scaffolds demonstrated its biocompatibility and potential use in tissue engineering.
