Autor(es): Pedroso, Silvia Cristina da Silva ; Lima, Karla Val?ria Batista ; Furlaneto, Ismari Perini ; Rodrigues, Yan Corr?a ; Pantoja, Darlene K?ssia Saraiva Queiroz ; Souza, Alex Junior Souza de ; Pereira, Washington Luiz Assun??o
Data: 2021
Origem: Oasisbr
Assunto(s): Tuberculose / veterin?ria; Tuberculose Bovina / transmiss?o; Mycobacterium bovis / patogenicidade; Infec??es por Mycobacterium / veterin?ria; Vigil?ncia em Sa?de P?blica; Inspe??o Sanit?ria; Zoonoses Bacterianas
This work was supported by the Instituto Evandro Chagas, Secretaria de Vigil?ncia em Sa?de, Minist?rio da Sa?de. Funda??o de Amparo ? Pesquisa do Par?/Universidade do Estado do Par? (FAPESPA/UEPA) [Cooperation grant N? 004/2019].
Federal University of Par?. Post-graduate Program in Animal Science. Bel?m, PA, Brazil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Laborat?rio de Cultura Celular e Citogen?tica. Ananindeua, PA, Brasil / State University of Par?. Post-graduate Program in Parasitic Biology in the Amazon. Bel?m, PA, Brazil.
State University of Par?. Post-graduate Program in Parasitic Biology in the Amazon. Bel?m, PA, Brazil / University Center of Par?. Post-graduate Program in Health Education - Medical Education. Bel?m, PA, Brazil.
State University of Par?. Post-graduate Program in Parasitic Biology in the Amazon. Bel?m, PA, Brazil.
Federal Rural University of Amazonia. Post-graduate Program in Animal Health and Production in the Amazon. Bel?m, PA, Brazil.
Max Planck University Center. Indaiatuba, SP, Brazil.
Federal Rural University of Amazonia. Institute of Animal Health and Production. Bel?m, PA, Brazil.
Introduction: Zoonotic tuberculosis is a disease of public health importance worldwide, especially in developing countries. The present study aimed to investigate the role played by Mycobacterium bovis and other mycobacteria as etiologic agents of bubaline tuberculosis (TB) in the Brazilian Amazon region. Methodology: Granulomatous lesions suggestive of TB obtained from 109 buffaloes (n =109) during sanitary inspection at slaughter were subjected to histopathological evaluation, immunohistochemical (IHC) detection of Mycobacterium antigens, and to molecular tests (PCR) to detect hsp65, IS6110 and RD4 genes, which are specific to Mycobacterium spp., Mycobacterium tuberculosis Complex (MTBC) and M. bovis, respectively. Results: PCR results indicated Mycobacterium infection in 87.2% of the cases, of which 69.5% were positive for M. bovis, 27.4% belonged to MTBC, and 3.1% were probably non-TB mycobacteria. There was good agreement between the genus-specific molecular technique and the histopathological analysis. This high frequency of TB cases caused by non-M. bovis suggests a diversified scenario of mycobacteria associated with bubaline TB in the Brazilian Amazon region.Conclusions: The results reinforce the need of discussing the inclusion of more accurate techniques in examinations carried out by Inspection Services in Brazil
