Autor(es): Queiroz, Alice Louize Nunes ; Barros, Rafael S ; Silva, Sandro Patroca da ; Rodrigues, Daniela Sueli Guerreiro ; Cruz, Ana Cec?lia Ribeiro ; Santos, Fl?via B. dos ; Vasconcelos, Pedro Fernando da Costa ; Tesh, Robert B ; Nunes, Bruno Tardelli Diniz
Data: 2021
Origem: Oasisbr
Assunto(s): Febre Amarela / patologia; Vacina contra Febre Amarela / efeitos adversos; Rea??o em Cadeia da Polimerase / m?todos; Epidemias
We are grateful to the National Council for Scientific and Technologic Development Agency-CNPq (processes 306581/2016-7 and 303999/2016-0) for financial support of the study
Minist?rio da Sa?de. Funda??o Servi?os de Sa?de P?blica. Instituto Evandro Chagas. Bel?m, PA, Brasil.
Minist?rio da Sa?de. Funda??o Servi?os de Sa?de P?blica. Instituto Evandro Chagas. Bel?m, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Funda??o Servi?os de Sa?de P?blica. Instituto Evandro Chagas. Bel?m, PA, Brasil.
Oswaldo Cruz Institute. Viral Immunology Laboratory. Rio de Janeiro, RJ, Brazil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
University Texas Medical Branch. Department of Pathology and Microbiology & Immunology. Galveston, TX, USA.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Abstract: From 2016 to 2018, Brazil faced the biggest yellow fever (YF) outbreak in the last 80 years, representing a risk of YF reurbanization, especially in megacities. Along with this challenge, the mass administration of the fractionated YF vaccine dose in a na?ve population brought another concern: the possibility to increase YF adverse events associated with viscerotropic (YEL-AVD) or neurological disease (YEL-AND). For this reason, we developed a quantitative real time RT-PCR (RT-qPCR) assay based on a duplex TaqMan protocol to distinguish broad-spectrum infections caused by wild-type yellow fever virus (YFV) strain from adverse events following immunization (AEFI) by 17DD strain during the vaccination campaign used to contain this outbreak. A rapid and more accurate RT-qPCR assay to diagnose YFV was established, being able to detect even different YFV genotypes and geographic strains that circulate in Central and South America. Moreover, after testing around 1400 samples from human cases, non-human primates and mosquitoes, we detected just two YEL-AVD cases, confirmed by sequencing, during the massive vaccination in Brazilian Southeast region, showing lower incidence than AEFI as expected.
