Author(s): Paz, Jasna Let?cia Pinto ; Silvestre, Maria do Perp?tuo Socorro Corr?a Amador ; Moura, Let?cia Siqueira ; Furlaneto, Ismari Perini ; Rodrigues, Yan Corr?a ; Lima, Karla Val?ria Batista ; Lima, Luana Nepomuceno Gondim Costa
Date: 2021
Origin: Oasisbr
Subject(s): Hansen?ase / patologia; Mycobacterium leprae / patogenicidade; Receptores de Calcitriol; Polimorfismo de Nucleot?deo ?nico / imunologia; T?cnicas de Genotipagem / m?todos; Gen?tipo; Biomarcadores / an?lise
Secretariat of Health Surveillance (SVS), Brazilian Ministry of Health (MS), Evandro Chagas Institute (IEC); Coordination for the Improvement of Higher Education Personnel (CAPES/Brazil) and National Council for Scientific and Technological Development (CNPq/Brazil).
State University of Para. Program in Parasitic Biology in the Amazon Region. Belem, PA, Brazil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
University Center of the State of Para. Belem, PA, Brazil.
State University of Para. Program in Parasitic Biology in the Amazon Region. Belem, PA, Brazil / Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
State University of Para. Program in Parasitic Biology in the Amazon Region. Belem, PA, Brazil / Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
State University of Para. Program in Parasitic Biology in the Amazon Region. Belem, PA, Brazil / Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Programa de P?s-Gradua??o em Epidemiologia e Vigil?ncia em Sa?de. Ananindeua, PA, Brasil.
The transmission and evolution of leprosy depends on several aspects, including immunological and genetic factors of the host, as well as genetic factors of Mycobacterium leprae. The present study evaluated the association of single nucleotide polymorphisms (SNPs) on the FokI (rs2228570), TaqI (rs731236), ApaI (rs7975232) regions of the vitamin D receptor (VDR) gene with leprosy. A total of 405 individuals were evaluated, composed by groups of 100 multibacillary (MB) and 57 paucibacillary (PB) patients, and 248 healthy contacts. Blood samples were collected from patients and contacts. The genotyping was performed by sequencing of the interest regions. The alleles of the studied SNPs, and SNP FokI genotypes, were not associated with leprosy. For the SNP on TaqI region, the relationship between the tt genotype, and for the SNP ApaI, the AA genotype, revealed an association with susceptibility to MB form, while Aa genotype with protection. The extended genotypes AaTT and AaTt of ApaI and TaqI were associated with protection against MB form. Further studies analyzing the expression of the VDR gene and the correlation with its SNPs might help to clarify the role of polymorphisms on the immune response in leprosy.
