Author(s): Reis, Nadabe dos Santos ; Lessa, Ozana Almeida ; Pacheco, Clissiane Soares Viana ; Pereira, Norma Eliane ; Soares, Glêydison Amarante ; Silva, Erik Galvão Paranhos ; Oliveira, Julieta Rangel ; Franco, Marcelo
Date: 2020
Origin: Oasisbr
Subject(s): waste; solid-state fermentation; Box-Behnken planning.; waste; solid-state fermentation; Box-Behnken planning.
The objective of this study was to evaluate the use of cocoa shell as an alternative substrate for the production of endoglucanase and xylanase by Aspergillus orizae ATCC 10124 in solid-state fermentation. The fermentation assays were performed using cocoa shells humidified with distilled water. The parameters incubation temperature, water activity and fermentation time were optimized by using a Box-Behnken design. The maximum activities achieved for endoglucanases and xylanases were 0.846 and 0.945 U g-1 at 35°C, with a water activity equal to 0.9 and an incubation time of 96 hours.
The objective of this study was to evaluate the use of cocoa shell as an alternative substrate for the production of endoglucanase and xylanase by Aspergillus orizae ATCC 10124 in solid-state fermentation. The fermentation assays were performed using cocoa shells humidified with distilled water. The parameters incubation temperature, water activity and fermentation time were optimized by using a Box-Behnken design. The maximum activities achieved for endoglucanases and xylanases were 0.846 and 0.945 U g-1 at 35°C, with a water activity equal to 0.9 and an incubation time of 96 hours.
