Design of an electrochemical genosensor for the BDNF gene polymorphism sequence detection using an enzymatic labelled DNA probe

• Author(s): Caldevilla, Renato ; Morais, Stephanie ; Carvalho, Serafim ; Medeiros, Rui ; Delerue-Matos, Cristina ; Cruz, Agostinho ; Santos, Marlene ; Barroso, M. Fátima
• Date: 2023
• Persistent ID: http://hdl.handle.net/10400.22/23787
• Origin: Repositório Científico do Instituto Politécnico do Porto
• Subject(s): Chronoamperometry; Depression; Electrochemical genosensor
• Document Type: Conference object
• Language: English
• Contributor(s): REPOSITÓRIO P.PORTO

Description

The BDNF gene is associated with high degrees of variability in antidepressant treatments. The Val66Met polymorphism is widely known as a source of this variability, warranting growing interest in genotyping patients that undergo antidepressant treatment to better suit their needs. This paper reports on an electrochemical genosensing platform, based on gold electrodes, capable of detecting this polymorphism, through the use of synthetic enzymatic labelled DNA-probes for 2 different BDNF alleles. The sensor showed promising results, and its applicability to real samples is currently being tested.