Generation of an obese diabetic mouse model upon conditional Atrx disruption

• Author(s): Gaspar, Tiago Bordeira ; Jesus, Tito Teles ; Azevedo, Maria Teresa ; Macedo, Sofia ; Soares, Mariana Alves ; Martins, Rui Sousa ; Leite, Rúben ; Rodrigues, Lia ; Rodrigues, Daniela Ferreira ; Cardoso, Luís ; Borges, Inês ; Canberk, Sule ; Gärtner, Fátima ; Miranda-Alves, Leandro ; Lopes, José Manuel ; Soares, Paula ; Vinagre, João
• Date: 2023
• Persistent ID: http://hdl.handle.net/10400.22/25532
• Origin: Repositório Científico do Instituto Politécnico do Porto
• Subject(s): Atrx; Conditional mouse model; Endocrine dysfunction; Fatty pancreas; Pancreatic fatty replacement; Hyperglycaemia; Hepatic stetosis; Inflammageing; Obesity; Pdx1-Cre
• Document Type: Journal article
• Language: English
• Contributor(s): REPOSITÓRIO P.PORTO

Description

ATRX mutations occur in up to 17% of human pancreatic neuroendocrine tumours (PanNETs), and recent evidence points towards its inability to drive PanNET formation in mouse pancreas while predisposing individuals to inflammageing. Aiming to explore the additional non-tumourigenic consequences of Atrx deletion, we characterised an aged series of Atrx conditional disruption in β cells using the Pdx1 promoter. Homozygous mice (P.AtrxHOM) exhibited obesity, diabetes, glucose intolerance, and pancreatic adiposity at a higher extent than age- and sex-matched controls (P.AtrxWT).