Genetic diversity of mycosphaerella fijiensis in brazil analyzed using an ERIC-PCR marker

• Autor(es): Silva, G. F. ; Paixão, R. D.V. ; Queiroz, Casley B. ; Santana, Mateus Ferreira ; Souza, A. S. ; Sousa, Nelcimar Reis ; Hanada, Rogério Eiji ; Gasparotto, Luadir
• Data: 2020
• Origem: Oasisbr
• Assunto(s): Brasil; Cluster Analysis; Correlation Analysis; Dna Extraction; Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction Marker; Fungus Isolation; Gene Locus; Genetic Analysis; Genetic Marker; Polymorphism, Genetic; Genetic Variability; Haplotype; Microsatellite Marker; Mycosphaerella; Mycosphaerella Fijiensis; Photosynthesis; Polymorphism, Restriction Fragment Length; Polymorphism, Single Nucleotide; Transposon; Ascomycetes; Classification; Genetics; Nucleotide Sequence; Polymerase Chain Reaction; Procedures; Species Difference; Musa; Mycosphaerella Fijiensis; Pseudocercospora; Genetic Marker; Primer Dna; Ascomycota; Base Sequence; Brasil; Dna Primers; Genetic Markers; Polymerase Chain Reaction; Species Specificity
• Tipo de Documento: Artigo científico
• Idioma: Inglês

Descrição

The Enterobacterial repetitive intergenic consensus (ERIC) marker was used to analyze the genetic variability of Mycosphaerella fijiensis, the causative agent of Black Sigatoka disease in banana plants. A total of 123 isolates were used, which were divided into populations based on their original hosts and collection sites in Brazil. A total of 9 loci were amplified, 77.8% of which were found to be polymorphic. The genetic diversity found in the population was 0.20. Analysis of molecular variance (AMOVA) demonstrated that the highest level of genetic variation is within populations. Cluster analysis revealed three main groups in Brazil, with no correlation between geographic and genetic distance. © FUNPEC-RP