Autor(es):
Guimarães-Marques, Giselle Moura ; Batista, Jacqueline da Silva ; Guimarães, H. M. ; Naice-Daou, M. V. ; Lima, M. P. ; Formiga, Kyara M. ; Santos, Joselita Maria M. ; Lima, A. C. ; Rafael, Míriam Silva
Data: 2020
Origem: Oasisbr
Assunto(s): Microsatellite Dna; Animals; Anopheles; Disease Carrier; Dna Sequence; Gene; Gene Locus; Genetics; Genotype; Polymerase Chain Reaction; Species Difference; Animal; Anopheles; Genes, Insect; Genetic Loci; Genotype; Insect Vectors; Microsatellite Repeats; Polymerase Chain Reaction; Sequence Analysis, Dna; Species Specificity
Descrição
The Anopheles albitasis complex includes 6 species, and 3 are considered as malaria vectors in Brazil. Twenty-five polymorphic microsatellite DNA loci were isolated and characterized in 24-36 individuals from the neighborhood of Puraquequara, Manaus, Amazonas State, Brazil. The number of estimated alleles ranged from 2 to 10, the observed heterozygosity ranged from 0.182 to 0.897, and the expected heterozygosity ranged from 0.260 to 0.854. Eleven loci showed significant deviation from Hardy-Weinberg equilibrium. Eleven loci were cross-amplified successfully in 5 Anopheles species. These microsatellite loci will be useful in studies investigating population structure and evolutionary genetics in A. albitarsis sensu lato and other A. albitarsis complex species.
