Improvement of a PCR test to diagnose infection by Mansonella ozzardi

• Autor(es): Vera, Luana Janaína Souza ; Basano, Sérgio de Almeida ; Camargo, Juliana de Souza Almeida Aranha ; França, Andonai Krauze de ; Godoi Matos Ferreira, Ricardo de ; Casseb, Almeida Andrade ; Medeiros, Jansen Fernandes de ; Fontes, Gilberto ; Camargo, Luis Marcelo Aranha
• Data: 2020
• Origem: Oasisbr
• Assunto(s): Blood Smear; Clinical Clinical Effectiveness; Controlled Study; Cost Effectiveness Analysis; Diagnostic Test; Diagnostic Test Accuracy Study; Giemsa Stain; Gold Standard; Human; Intermethod Comparison; Mansonella Ozzardi; Mansonelliasis; Membrane Filter; Nonhuman; Nucleotide Sequence; Performance Measurement System; Polycarbonate Membrane Filter; Polymerase Chain Reaction; Predictive Value; Sensitivity And Specificity; Mansonella Ozzardi; Nucleopolyhedrovirus
• Tipo de Documento: Artigo científico
• Idioma: Português

Descrição

Introduction: Mansonelliasis is caused by Mansonella ozzardi. It is widespread in the Amazon region, with a high prevalence. Te common exam of thick blood smears stained with Giemsa shows low efficacy levels and has been an obstacle to diagnosing individuals with low blood parasitemia. Methods: In order to increase diagnosis efcacy, the PCR technique was improved. Results and Conclusions: PCR demonstrated the best performance, with sensitivity and negative predictive values (NPV) of 100%, followed by blood fltration through membrane filters, which showed a sensitivity of 88.9% and a NPV of 84.6%, when compared to thick blood smears.