A novel vasorelaxant lectin purified from seeds of Clathrotropis nitida: Partial characterization and immobilization in chitosan beads

• Autor(es): Alves, Ana Cecília ; Vasconcelos, Mayron Alves de ; Santiago, Mayara Quiroz ; Pinto-Junior, Vanir Reis ; Osterne, Vinicius José Silva ; Lóssio, Cláudia Figueiredo ; Souza Ferreira Bringel, Pedro Henrique ; Castro, Rondinelle Ribeiro ; Nagano, C. S. ; Delatorre, Plínio ; Souza, Luis Augusto Gomes ; Nascimento, K. S. ; Assreuy, Ana Maria Sampaio ; Cavada, B. S.
• Data: 2020
• Origem: Oasisbr
• Assunto(s): Calcium; Carbohydrate; Chitosan; Galactoside; Lectin; Magnesium; Stomach Mucin; Immobilized Protein; Plant Lectin; Vasodilator Agent; Animals Cell; Animals Experiment; Animals Model; Binding Affinity; Carbohydrate Analysis; Clathrotropis Nitida; Controlled Study; Dose Response; Electrospray Mass Spectrometry; Glycobiology; Male; Medicinal Plant; Molecular Weight; Nonhuman; Physical Chemistry; Seed Plant; Electrophoresis, Polyacrylamide Gel; Priority Journal; Protein Analysis; Protein Immobilization; Rat; Temperature; Vascular Ring; Vasodilatation; Amino Acid Sequence; Animals; Artemia; Chemistry; Drug Effects; Fabaceae; Genetics; Hemagglutination; Human; In Vitro Study; Isolation And Purification; Molecular Genetics; Physiology; Rabbit; Sequence Homology; Aorta, Thoracic; Wistar Rat; Amino Acid Sequence; Animal; Aorta, Thoracic; Artemia; Chitosan; Fabaceae; Hemagglutination; Humans; Immobilized Proteins; In Vitro Techniques; Male; Molecular Sequence Data; Molecular Weight; Plant Lectins; Plants, Medicinal; Rabbits; Rats; Rats, Wistar; Seeds; Sequence Homology, Amino Acid; Vasodilator Agents
• Tipo de Documento: Artigo científico
• Idioma: Inglês

Descrição

A novel lectin from seeds of Clathrotropis nitida (CNA) was purified and characterized. CNA is a glycoprotein containing approximately 3.3% carbohydrates in its structure. CNA promoted intense agglutination of rabbit erythrocytes, which was inhibited by galactosides and porcine stomach mucin (PSM). The lectin maintained its hemagglutinating activity after incubation in a wide range of temperatures (30-60 °C) and pH (6.0-7.0), and its binding activity was dependent on divalent cations (Ca+2 and Mg+2). SDS-PAGE showed an electrophoretic profile consisting of a single band of 28 kDa, as confirmed by electrospray ionization mass spectrometry, which indicated an average molecular mass of 27,406 ± 2 Da and the possible presence of isoforms and glycoforms. In addition, CNA exhibited no toxicity to Artemia sp. nauplii and elicited reversible and dose-dependent vasorelaxation in precontracted aortic rings. CNA was successfully immobilized on chitosan beads and was able to capture PSM in solution. This study demonstrated that CNA is a lectin that has potential as a biotechnological tool in glycomics and glycoproteomics applications. © 2015 Elsevier Inc.