Author(s):
Mesquita, Denny Willian Oliveira ; Mesquita, Adriana Spirotto Stein ; Cursino, Lorena Mayara de Carvalho ; Souza, E. S. ; Oliveira, A C ; Pinheiro, Carlos Cleomir S. ; Novaes, J. A P ; Oliveira, J. A.A. ; Nunez, C. V.
Date: 2020
Origin: Oasisbr
Subject(s): Duroia Saccifera Extract; Ferdinandusa Goudotiana Extract; Ferdinandusa Hirsuta Extract; Ferdinandusa Paraensis Extract; Palicourea Corymbifera Extract; Palicourea Guianensis Extract; Unclassified Drug; Abts Radical Scavenging Assay; Agar Diffusion; Animals Model; Anti-fungal Activity; Antioxidant Activity; Artemia Salina; Arthroderma Gypseum; Arthroderma Otae; Biological Activity; Candida Albicans; Cladosporium Cladosporioides; Cladosporium Sphaerospermum; Cytotoxicity; Dpph Radical Scavenging Assay; Duroia Saccifera; Epidermophyton Floccosum; Ferdinandusa Goudotiana; Ferdinandusa Hirsuta; Ferdinandusa Paraensis; Fusarium; Nonhuman; Palicourea Corymbifera; Palicourea Guianensis; Plant Leaf; Rubiaceae; Scytalidium; Toxicity Testing; Trichophyton Mentagrophytes; Trichophyton Rubrum; Trichophyton Tonsurans
Description
Rubiaceae is a plant family with great chemical and biological potential. In this work a phytochemical prospection was performed and it was assessed the cytotoxic, antioxidant and antifungal activities of dichloromethanic and methanolic extracts of the leaves of Duroia saccifera, Ferdinandusa goudotiana, F. hirsuta, F. paraensis, Ferdinandusa sp., Palicourea corymbifera and P. guianensis. The cytotoxicity evaluation was carried out using the test toxicity on Artemia salina. The antifungal activity was determined by agar diffusion method using fungi: Epidermophyton floccosum, Microsporum canis 32905, M. gipseum 29/00, Trichophyton mentagrophytes ATCC 9533/03, T. rubrum ATCC 28189, T. tonsurans 21/97, Cladosporium cladosporioides, C. sphaerospermum, Fusarium U. 662/06, Scytalidium U. 661/06, Candida albicans ATCC 3632 and C. albicans U. 5/99. The antioxidant activity was determined by testing the amount of free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) and the discoloration of the radical cation 6-sulfonic acid-2,2-azinobis-3-ethylbenzothiazoline (ABTS). For the toxicity test on A. salina greater toxicity in the methanol extract of F. goudotiana was observed, which proved to be toxic up to a concentration of 5 μg mL-1. The presence of antifungal principles was observed in the F. hirsuta and F. paraensis against four tested fungi. In the evaluation of the antioxidant activity, the methanol extracts showed greater activity than the dichloromethanic ones, being similar the obtained result through both methods (reduction of DDPH and ABTS decolorization). The results create possibilities for future researches related to the structure and activity of the components of each active extract. © 2015, Instituto de Biociencias. All rights reserved.
