The Genome of Anopheles darlingi, the main neotropical malaria vector

• Autor(es): Marinotti, Osvaldo ; Cerqueira, Gustavo C. ; Almeida, Luiz G.P. ; Ferro, Maria Inês Tiraboschi ; Loreto, Elgion Lúcio da Silva ; Zaha, Arnaldo ; Teixeira, Santuza Ribeiro ; Wespiser, Adam R. ; e Silva, A. A. ; Schlindwein, Aline Daiane ; Pacheco, Ana Carolina Landim ; Silva, Artur ; Graveley, Brenton R. ; Walenz, Brian P. ; Araújo Lima, Bruna de ; Ribeiro, Carlos Alexandre Gomes ; Nunes-Silva, Carlos Gustavo ; Carvalho, Carlos Roberto de ; Almeida Soares, Célia Maria A. de ; Menezes, Cláudia Beatriz Afonso de ; Matiolli, Cleverson Carlos ; Caffrey, Daniel R. ; Araujo, Demetrius Antonio Machado ; Oliveira, Diana Magalhães de ; Golenbock, Douglas T. ; Grisard, Edmundo Carlos ; Fantinatti-Garboggini, Fabiana ; Carvalho, Fabíola Marques de ; Barcellos, Fernando Gomes ; Prosdocimi, Francisco ; May, Gemma ; Azevedo Júnior, Gilson Martins de ; Guimarães, Giselle Moura ; Goldman, Gustavo Henrique ; Padilha, Itácio Q.M. ; Batista, Jacqueline da Silva ; Ferro, Jesus Aparecido ; Ribeiro, J. M.C. ; Serrao, Jose Eduardo Lopes Rangel ; Dabbas, Karina Maia ; Cerdeira, Louise Teixeira ; Agnez-Lima, Lucymara Fassarella ; Brocchi, Marcelo ; Carvalho, Marcos Oliveira de ; Teixeira, Marcus de Melo ; Mascena Diniz Maia, Maria de ; Goldman, Maria Helena S. ; Schneider, Maria Paula Cruz ; Felipe, Maria Sueli Soares ; Hungría, Mariangela ; Nicolás, Marisa Fabiana ; Pereira, Maristela ; Montes, Martín Alejandro ; Cantão, Maurício Egídio ; Vincentz, Michel G.A. ; Rafael, Míriam Silva ; Silverman, Neal ; Stoco, Patrícia Hermes ; Souza, Rangel Celso ; Vicentini, Renato ; Gazzinelli, Ricardo Tostes Ostes ; Oliveira Neves, Rogério de ; Silva, Rosane A. ; Astolfi-Filho, Spártaco A.T. ; Maciel, Talles Eduardo Ferreira ; Ürményi, Turán Péter ; Tadei, Wanderli Pedro ; Camargo, Erney Plessmann ; Vasconcelos, Ana Tereza
• Data: 2020
• Origem: Oasisbr
• Assunto(s): Anopheles; Anopheles Darlingi; Anopheles Gambiae; Circadian Rhythm; Female; Gene Identification; Gene Sequence; Genetic Conservation; Genetic Marker; Genome Analysis; Genome Size; Indel Mutation; Insect Genetics; Insecticide Resistance; Male; Nonhuman; Priority Journal; Sequence Homology; Polymorphism, Single Nucleotide; Synteny; Transposon; Xenobiotic Metabolism; Animal; Anopheles; Brasil; Chromosomes, Insect; Dna Transposable Elements; Evolution, Molecular; Female; Genetic Variation; Genome, Insect; Host-parasite Interactions; Insect Proteins; Insect Vectors; Insecticide Resistance; Insecticides; Malaria; Male; Molecular Sequence Annotation; Phylogeny; Synteny; Transcriptome
• Tipo de Documento: Artigo científico
• Idioma: Inglês

Descrição

Anopheles darlingi is the principal neotropical malaria vector, responsible for more than a million cases of malaria per year on the American continent. Anopheles darlingi diverged from the African and Asian malaria vectors ∼100 million years ago (mya) and successfully adapted to the New World environment. Here we present an annotated reference A. darlingi genome, sequenced from a wild population of males and females collected in the Brazilian Amazon. A total of 10 481 predicted protein-coding genes were annotated, 72% of which have their closest counterpart in Anopheles gambiae and 21% have highest similarity with other mosquito species. In spite of a long period of divergent evolution, conserved gene synteny was observed between A. darlingi and A. gambiae. More than 10 million single nucleotide polymorphisms and short indels with potential use as genetic markers were identified. Transposable elements correspond to 2.3% of the A. darlingi genome. Genes associated with hematophagy, immunity and insecticide resistance, directly involved in vectorhuman and vectorparasite interactions, were identified and discussed. This study represents the first effort to sequence the genome of a neotropical malaria vector, and opens a new window through which we can contemplate the evolutionary history of anopheline mosquitoes. It also provides valuable information that may lead to novel strategies to reduce malaria transmission on the South American continent. The A. darlingi genome is accessible at www.labinfo.lncc.br/index.php/anopheles- darlingi. © 2013 The Author(s).