Author(s):
Wiegand, S ; Jogler, M ; Boedeker, C ; Pinto, D ; Vollmers, J ; Rivas-Marín, E ; Kohn, T ; Peeters, S.H ; Heuer, A ; Rast, P ; Oberbeckmann, S ; Bunk, B ; Jeske, O ; Meyerdierks, A ; Storesund, J.E ; Kallscheuer, N ; Lücker, S ; Lage, O.M ; Pohl, T ; Merkel, B.J ; Hornburger, P ; Müller, R.W ; Brümmer, F ; Labrenz, M ; Spormann, A.M ; Op den Camp, H.J.M ; Overmann, J ; Amann, R ; Jetten, M.S.M ; Mascher, T ; Medema, M.H ; Devos, D.P ; Kaster, A.K ; Øvreås, L ; Rohde, M ; Galperin, M.Y ; Jogler, C.
Date: 2019
Persistent ID: https://hdl.handle.net/10216/130476
Origin: Repositório Aberto da Universidade do Porto
Subject(s): amino acid analysis; aquatic environment; Article; bacterial cell; bacterial genome; bacterial strain; bacterial structures; bacterium culture; bacterium identification; cell division; cell structure; cladistics; cytology; electron microscopy; gene sequence; genetic analysis; microbial diversity; nonhuman; Planctomycetes; priority journal; signal transduction; species habitat; taxonomy; bacterial phenomena and functions; bacterium; classification; ecosystem; genetic variation; genetics; growth, development and aging; phylogeny; secondary metabolism; RNA 16S; Bacteria; Bacterial Physiological Phenomena; Cell Division; Ecosystem; Genetic Variation; Genome, Bacterial; Phylogeny; RNA, Ribosomal, 16S; Secondary Metabolism; Signal Transduction
Description
When it comes to the discovery and analysis of yet uncharted bacterial traits, pure cultures are essential as only these allow detailed morphological and physiological characterization as well as genetic manipulation. However, microbiologists are struggling to isolate and maintain the majority of bacterial strains, as mimicking their native environmental niches adequately can be a challenging task. Here, we report the diversity-driven cultivation, characterization and genome sequencing of 79 bacterial strains from all major taxonomic clades of the conspicuous bacterial phylum Planctomycetes. The samples were derived from different aquatic environments but close relatives could be isolated from geographically distinct regions and structurally diverse habitats, implying that ‘everything is everywhere’. With the discovery of lateral budding in ‘Kolteria novifilia’ and the capability of the members of the Saltatorellus clade to divide by binary fission as well as budding, we identified previously unknown modes of bacterial cell division. Alongside unobserved aspects of cell signalling and small-molecule production, our findings demonstrate that exploration beyond the well-established model organisms has the potential to increase our knowledge of bacterial diversity. We illustrate how ‘microbial dark matter’ can be accessed by cultivation techniques, expanding the organismic background for small-molecule research and drug-target detection. © 2019, The Author(s), under exclusive licence to Springer Nature Limited.
