Autor(es): Pedro, ARV ; Lima, T ; Fróis-Martins, R ; Leal, B ; Ramos, IC ; Martins, EG ; Cabrita, ARJ ; Fonseca, AJM ; Maia, MRG ; Vilanova, M ; Correia, A
Data: 2021
Identificador Persistente: https://hdl.handle.net/10216/149489
Origem: Repositório Aberto da Universidade do Porto
Assunto(s): CLEC7A; bovine; cytokines; dectin-1; monocytes; siRNA; β-glucans
Yeast-derived products containing β-glucans have long been used as feed supplements in domesticated animals in an attempt to increase immunity. β-glucans are mainly recognized by the cell surface receptor CLEC7A, also designated Dectin-1. Although the immune mechanisms elicited through Dectin-1 activation have been studied in detail in mice and humans, they are poorly understood in other species. Here, we evaluated the response of bovine monocytes to soluble and particulate purified β-glucans, and also to Zymosan. Our results show that particulate, but not soluble β-glucans, can upregulate the surface expression of costimulatory molecules CD80 and CD86 on bovine monocytes. In addition, stimulated cells increased production of IL-8 and of TNF, IL1B, and IL6 mRNA expression, in a dose-dependent manner, which correlated positively with CLEC7A gene expression. Production of IL-8 and TNF expression decreased significantly after CLEC7A knockdown using two different pairs of siRNAs. Overall, we demonstrated here that bovine monocytes respond to particulate β-glucans, through Dectin-1, by increasing the expression of pro-inflammatory cytokines. Our data support further studies in cattle on the induction of trained immunity using dietary β-glucans.
