Recovery of ergosterol from Agaricus bisporus mushrooms via supercritical fluid extraction: A response surface methodology optimisation

• Author(s): Almeida C.F. ; Manrique, Y.A. ; Lopes J.C.B. ; Martins, FG ; Madalena M. Dias
• Date: 2024
• Persistent ID: https://hdl.handle.net/10216/162609
• Origin: Repositório Aberto da Universidade do Porto
• Document Type: Journal article
• Language: English

Description

This work uses the response surface methodology to optimise ergosterol recovery from Agaricus bisporus mushrooms by supercritical fluid extraction. The influence of pressure, temperature, co-solvent use, and solvent-to-mushroom mass ratio was evaluated on yield and extract composition. Considering temperature, pressure, and co-solvent volume percentage, predictive models were regressed for extraction yield, ergosterol purity in the recovered fractions, and ergosterol recovery. The co-solvent volumetric fraction was the most influential factor, increasing the extraction yield and reducing the extract's ergosterol purity. A maximum ergosterol extract purity of (547.27 ± 2.37) mgergosterol·gextract1 was obtained at a pressure of 100 bar, temperature of 50 °C and co-solvent volume percentage of 5 % v/v. The highest ergosterol recovery was (6.23 ± 0.06) mgergosterol·gdw1 for operating conditions of 244 bar, 56 °C, and 8 % v/v of co-solvent. Coefficients of determination close to one were obtained for all models, indicating good agreement with experimental data.