Growth kinetics of mycobacterium tuberculosis measured by quantitative resazurin reduction assay: a tool for fitness studies

• Autor(es): Groll, Andrea Von ; Martin, Anandi ; Portaels, Françoise ; Silva, Pedro Eduardo Almeida da ; Palomino, Juan Carlos
• Data: 2012
• Origem: Oasisbr
• Assunto(s): Growth kinetics; Mycobacterium tuberculosis; Resazurin; Fitness
• Tipo de Documento: Artigo científico
• Idioma: Inglês

Descrição

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We standardized a method to evaluate the growth kinetics of Mycobacterium tuberculosis by measuring quantitatively the reduction of resazurin by spectrophotometry. Growth curves and the rate of growth of twenty-one M. tuberculosis clinical isolates were determined. The method showed technical simplicity and is inexpensive to assess the fitness of each isolate.