Growth kinetics of mycobacterium tuberculosis measured by quantitative resazurin reduction assay: a tool for fitness studies

• Author(s): Groll, Andrea Von ; Martin, Anandi ; Portaels, Françoise ; Silva, Pedro Eduardo Almeida da ; Palomino, Juan Carlos
• Date: 2012
• Origin: Oasisbr
• Subject(s): Growth kinetics; Mycobacterium tuberculosis; Resazurin; Fitness
• Document Type: Journal article
• Language: English

Description

Submitted by Michele Fernanda ([e-mail]) on 2012-09-18T00:48:59Z No. of bitstreams: 1 Growth kinetics of Mycobacterium tuberculosis measured by quantitative resazurin reduction assay- a tool for fitness studies.pdf: 157755 bytes, checksum: 5421c0c4b06ffb88b69154be5f0936c8 (MD5)

Approved for entry into archive by gabriela rosa([e-mail]) on 2012-09-18T01:19:15Z (GMT) No. of bitstreams: 1 Growth kinetics of Mycobacterium tuberculosis measured by quantitative resazurin reduction assay- a tool for fitness studies.pdf: 157755 bytes, checksum: 5421c0c4b06ffb88b69154be5f0936c8 (MD5)

Made available in DSpace on 2012-09-18T01:19:15Z (GMT). No. of bitstreams: 1 Growth kinetics of Mycobacterium tuberculosis measured by quantitative resazurin reduction assay- a tool for fitness studies.pdf: 157755 bytes, checksum: 5421c0c4b06ffb88b69154be5f0936c8 (MD5) Previous issue date: 2010

We standardized a method to evaluate the growth kinetics of Mycobacterium tuberculosis by measuring quantitatively the reduction of resazurin by spectrophotometry. Growth curves and the rate of growth of twenty-one M. tuberculosis clinical isolates were determined. The method showed technical simplicity and is inexpensive to assess the fitness of each isolate.