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Microcolony detection in thin layer culture as an alternative method for rapid detection of mycobacterium tuberculosis in clinical samples.

Author(s): Silva, Pedro Eduardo Almeida da ; Wiesel, Fernanda ; Boffo, Maria Marta Santos ; Groll, Andrea Von ; Mattos, Ivo Gomes de ; Mejia, Glória ; Robledo, Jaime

Date: 2012

Origin: Oasisbr

Subject(s): Tuberculosis; Rapid diagnosis; Mycobacterium tuberculosis; Tuberculose; Diagnóstico rápido


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Tuberculosis remains one of the major causes of mortality worldwide. The early detection of new cases is an important goal in the program of tuberculosis control. Several methodologies for rapid and accurate laboratorial diagnosis have been developed, however, some of these techniques are expensive or cumbersome, making their implementation in low-income regions unfeasible. In this study, the thin layer culture method was compared with conventional culture method and it was observed that it provides earlier results and a presumptive species identification, being adequate alternative method for rapid laboratory diagnosis.

A tuberculose permanece como uma das principais causas de mortalidade em todo o mundo. A detecção precoce dos casos novos é um importante ponto nos programas de controle da tuberculose. Diversas metodologias para um rápido e acurado diagnóstico laboratorial tem sido desenvolvidas, entretanto, algumas destas técnicas são de alto custo ou de difícil execução, tornando inexeqüível sua implementação em locais com poucos recursos. Neste trabalho o método de cultivo em camada delgada foi comparado ao método de cultivo convencional verificandose apresenta resultados positivos em menor tempo com uma identificação presuntiva da espécie de micobactéria, sendo assim um método alternativo adequado para o diagnóstico laboratorial rápido.

Document Type Journal article
Language English
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