Direct application of the INNO-LiPA Rif.TB line-probe assay for rapid identification of Mycobacterium tuberculosis complex strains and detection of rifampin resistance in 360 Mycobacterium tuberculosis complex strains and detection of rifampin resistance in 360 smear-positive respiratory specimens from an area of high incidence of multidrug-resistant tuberculosis

• Author(s): Viveiros, M ; Leandro, C ; Rodrigues, L ; Almeida, J ; Bettencourt, R ; Couto, I ; Carrilho, L ; Diogo, J ; Fonseca, A ; Lito, L ; Lopes, J ; Pacheco, T ; Pessanha, M ; Quirim, J ; Sancho, L ; Salfinger, M ; Amaral, L
• Date: 2005
• Persistent ID: http://hdl.handle.net/10400.10/416
• Origin: Repositório do Hospital Prof. Doutor Fernando Fonseca
• Subject(s): Tuberculose; Tuberculose pulmonar; Portugal; Mycobacterium tuberculosis; Multidrug-resistant tuberculosis
• Document Type: Journal article
• Language: English
• Contributor(s): Unidade Local de Saúde Amadora / Sintra

Description

The INNO-LiPA Rif.TB assay for the identification of Mycobacterium tuberculosis complex strains and the detection of rifampin (RIF) resistance has been evaluated with 360 smear-positive respiratory specimens from an area of high incidence of multidrug-resistant tuberculosis (MDR-TB). The sensitivity when compared to conventional identification/culture methods was 82.2%, and the specificity was 66.7%; the sensitivity and specificity were 100.0% and 96.9%, respectively, for the detection of RIF resistance. This assay has the potential to provide rapid information that is essential for the effective management of MDR-TB.