Plasmid profile analysis of Portuguese Borrelia lusitaniae strains

• Author(s): Vitorino, Liliana ; Margos, Gabriele ; Zé-Zé, Líbia ; Kurtenbach, Klaus ; Collares-Pereira, Margarida
• Date: 2010
• Persistent ID: http://hdl.handle.net/10400.18/183
• Origin: Repositório Científico do Instituto Nacional de Saúde
• Subject(s): Lyme borreliosis; Borrelia lusitaniae; Genome organization; ospA; Ixodes ricinus; Estudos de Vectores e Doenças Infecciosas
• Document Type: Journal article
• Language: English
• Contributor(s): Repositório Científico do Instituto Nacional de Saúde

Description

Plasmid profilesof2Portuguese Borrelia lusitaniae strains, oneisolatedfromahumanpatientandthe other onefroman Ixodes ricinus tick, wereobtainedbypulsed-fieldgelelectrophoresistoevaluatethe plasmid diversityineachstrain.Overall,amaximumof6plasmidsweredetectedthatrangedfrom 19 kbto76kb,revealingcompletelydifferentplasmidprofilesfromthosepreviouslydescribedforother genospecies of B. burgdorferi sensu lato,thecausativeagentsofLymeborreliosis.Theplasmidlocationof the ospA gene wasinvestigatedbyhybridization,allowingitsallocationtotheplasmidof70kbinstead of the54kblinearplasmiddescribedfor B. burgdorferi sensu strictostrains.