Author(s): Vaz-Moreira, Ivone ; Narciso-da-Rocha, Carlos ; De Brandt, Evie ; Vandamme, Peter ; Ferreira, A. C. Silva ; Lobo-da-Cunha, Alexandre ; Nunes, Olga C. ; Manaia, Célia M.
Date: 2015
Persistent ID: http://hdl.handle.net/10400.14/25557
Origin: Veritati - Repositório Institucional da Universidade Católica Portuguesa
An aerobic, Gram-stain-negative rod, designated strain A2P5T , was isolated from the Douro river, in Porto, Portugal. Cells were catalase- and oxidase-positive. Growth occurred at15–30 8C, at pH 6–8 and in the presence of 1 % (w/v) NaCl. The major respiratory quinone was Q8, the genomic DNA had a G+C content of 47¡1 mol%, and phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol were amongst the major polar lipids. On the basis of 16S rRNA gene sequence analysis, strain A2P5T was observed to be a member of the family Burkholderiaceae, but could not be identified as a member of any validly named genus. The low levels of 16S rRNA gene sequence similarity to other recognized taxa (,91 %), together with the comparative analysis of phenotypic and chemotaxonomic characteristics, supported the proposal of a novel species of a new genus within the family Burkholderiaceae. The name Hydromonas duriensis gen. nov., sp. nov. is proposed. The type strain of Hydromonas duriensis is A2P5T (5LMG 28428T 5CCUG 66137T).
