Anti-leishmanial ige antibodies: a marker of active disease in visceral leishmaniasis

• Author(s): Atta, Ajax Mercês ; D'Oliveira Junior, Argemiro ; Correa, Jefferson ; Atta, Maria Luiza Brito de Sousa ; Almeida, Roque Pacheco de ; Carvalho Filho, Edgar Marcelino de ; Atta, Ajax Mercês ; D'Oliveira Junior, Argemiro ; Correa, Jefferson ; Atta, Maria Luiza Brito de Sousa ; Almeida, Roque Pacheco de ; Carvalho Filho, Edgar Marcelino de
• Date: 2013
• Origin: Oasisbr
• Subject(s): Leishmaniose visceral
• Document Type: Journal article
• Language: English

Description

p. 426–430

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Abstract. Visceral leishmaniasis (VL) is characterized by a depression of the T helper cell type 1 immune response. Although mRNA expression for interleukin-4 (IL-4) is observed, evidence of the role of this cytokine in the pathogenesis of VL has been lacking. Since IL-4 is involved in IgE synthesis, we measured the total IgE and Leishmania antigen-specific IgE antibody levels in sera from patients with VL. Specific IgE antibodies detected by an ELISA technique after absorbing the sera with purified sheep IgG anti-human IgG were found in all 23 patients with VL and were not detected in subjects with subclinical Leishmania chagasi infection (n 5 10), Chagas’ disease (n 5 10), atopic patients (n 5 10), and healthy controls (n 5 10). Levels of Leishmania-specific IgE (optical density values) before and after treatment were 0.100 6 0.03 (mean 6 SD) and 0.028 6 0.002, respectively (P , 0.05). These results indicate that a specific IgE response is useful in the diagnosis of active disease and to evaluate response to treatment.

Salvador