Molecular characterization of the Corynebacterium pseudotuberculosis hsp60-hsp10 operon, and evaluation of the immune response and protective efficacy induced by hsp60 DNA vaccination in mice

• Autor(es): Almeida, Sintia Silva de ; Costa, Marcilia P. ; Dorella, Fernanda Alves ; Oliveira, Sérgio Costa ; Miyoshi, Anderson ; Azevedo, Vasco Ariston de Carvalho ; McCulloch, John Anthony ; Fonseca, Cristina Toscano ; Oliveira, Diana Magalhães de ; Teixeira, Maria Fátima da Silva ; Laskowska, Ewa ; Almeida, Sintia Silva de ; Costa, Marcilia P. ; Dorella, Fernanda Alves ; Oliveira, Sérgio Costa ; Miyoshi, Anderson ; Azevedo, Vasco Ariston de Carvalho ; McCulloch, John Anthony ; Fonseca, Cristina Toscano ; Oliveira, Diana Magalhães de ; Teixeira, Maria Fátima da Silva ; Laskowska, Ewa
• Data: 2014
• Origem: Oasisbr
• Tipo de Documento: Artigo científico
• Idioma: Inglês

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Background Heat shock proteins (HSPs) are important candidates for the development of vaccines because they are usually able to promote both humoral and cellular immune responses in mammals. We identified and characterized the hsp60-hsp10 bicistronic operon of the animal pathogen Corynebacterium pseudotuberculosis, a Gram-positive bacterium of the class Actinobacteria, which causes caseous lymphadenitis (CLA) in small ruminants. Findings To construct the DNA vaccine, the hsp60 gene of C. pseudotuberculosis was cloned in a mammalian expression vector. BALB/c mice were immunized by intramuscular injection with the recombinant plasmid (pVAX1/hsp60). Conclusion This vaccination induced significant anti-hsp60 IgG, IgG1 and IgG2a isotype production. However, immunization with this DNA vaccine did not confer protective immunity.