Autor(es): Carvalho, Rusbene Bruno Fonseca de ; Almeida, Antonia Amanda Cardoso de ; Freitas, Rivelilson Mendes de ; Lima, Luciano Silva ; David, Juceni Pereira de Lima ; David, Jorge Mauricio ; Feitosa, Chistiane Mendes
Data: 2014
Origem: Oasisbr
Assunto(s): Citrus limon (L.) Burm; Psoraleno; Acetylcholinesterase
p. 1375-1379
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Made available in DSpace on 2014-03-20T13:34:38Z (GMT). No. of bitstreams: 1 0100-40422013000900017.pdf: 148624 bytes, checksum: 42e8b800982fb8e3600bed1d6ca8509f (MD5) Previous issue date: 2013
This paper describes the chemical composition and acetylcholinesterase inhibition of an active chromatographic fraction of the EtOAc extract of Citrus limon leaves. The composition of the active fraction presented a mixture of two coumarins, 5,8-dimethoxy-psoralen and 5,7- dimethoxycoumarin, identified by ¹H and ¹³C NMR data analysis, including DEPT, COSY, HMBC and HSQC experiments. It was also demonstrated that this mixture presents qualitative and quantitative AChE inhibition. In vitro studies indicated a CE50 value of 340 µg/mL with 95% of confidence. In vivo studies (10 and 25 mg/kg) revealed inhibition of 30.09 and 30.06% of AChE activity in relation to neostigmine, respectively.
