Author(s):
Barreto, Mauricio Lima ; FrotaI, Cristiane Cunha ; Lima, Luana Nepomuceno Costa ; Rocha, Adalgiza da Silva ; Suffys, Philip Noel ; Rolim, Benedito Neilson ; Rodrigues, Laura Cunha ; Kendall, Carl Kendall ; Kerr, Ligia Regina Franco Sansigolo ; Barreto, Mauricio Lima ; FrotaI, Cristiane Cunha ; Lima, Luana Nepomuceno Costa ; Rocha, Adalgiza da Silva ; Suffys, Philip Noel ; Rolim, Benedito Neilson ; Rodrigues, Laura Cunha ; Kendall, Carl Kendall ; Kerr, Ligia Regina Franco Sansigolo
Date: 2014
Origin: Oasisbr
Subject(s): Euphractus sexcinctus; Dasypus novemcinctus; Mycobacterium leprae; eco-epidemiology; Leprosy
Description
p. 209-213
Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2014-04-24T14:27:32Z No. of bitstreams: 1 Maurício Lima Barreto.pdf: 309292 bytes, checksum: 875893a79fdbacccb571c4631d28558c (MD5)
Made available in DSpace on 2014-04-24T14:27:33Z (GMT). No. of bitstreams: 1 Maurício Lima Barreto.pdf: 309292 bytes, checksum: 875893a79fdbacccb571c4631d28558c (MD5) Previous issue date: 2012
Human beings are the main reservoir of the causative agent of leprosy, Mycobacterium leprae. In the Americas, nine-banded armadillos (Dasypus novemcinctus) also act as a reservoir for the bacillus. In the state of Ceará (CE), which is located in Northeast Brazil and is an endemic area of leprosy, there are several species of armadillos, including D. novemcinctus and Euphractus sexcinctus (six-banded armadillo). Contact between humans and armadillos occur mainly through hunting, cleaning, preparing, cooking and eating. This study identified M. leprae DNA in the two main species of armadillos found in Northeast Brazil. A total of 29 wild armadillos (27 D. novemcinctus and 2 E. sexcinctus) were captured in different environments of CE countryside. Samples from the ear, nose, liver and spleen from each of these animals were tested by a nested M. leprae-specific repetitive element polymerase chain reaction assay. The samples that tested positive were confirmed by DNA sequencing. M. leprae was detected in 21% (6/29) of the animals, including five D. novemcinctus and one E. sexcinctus. This is the first Brazilian study to identify the presence of a biomarker of M. leprae in wild armadillos (D. novemcinctus and E. sexcinctus) in a leprosy hyperendemic area where there is continuous contact between humans and armadillos.
