Author(s):
Dantas, Marina Loyola ; Oliveira, Juliana Cabral de ; Carvalho, Lucas Pedreira de ; Passos, Sara Timóteo ; Queiroz, Adriano ; Machado, Paulo Roberto Lima ; Carvalho Filho, Edgar Marcelino de ; Arruda, Sérgio Marcos ; Dantas, Marina Loyola ; Oliveira, Juliana Cabral de ; Carvalho, Lucas Pedreira de ; Passos, Sara Timóteo ; Queiroz, Adriano ; Machado, Paulo Roberto Lima ; Carvalho Filho, Edgar Marcelino de ; Arruda, Sérgio Marcos
Date: 2014
Origin: Oasisbr
Subject(s): Leishmaniasis; Cytotoxicity; Inflammation; Cutaneous; CD8; Human
Description
p. 728-734
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Introduction: Leishmania braziliensis infection induces a large spectrum of lesions that clinically manifest as nodules or papules that progress to ulcers. Although it is already known that T helper cells predominate in the lesions, cytotoxic T cells have also been reported to be present, and their role in leishmaniasis immunopathogenesis is not well known. This study investigated the amounts of CD8+ and granzyme B+ cells in different clinical forms of human cutaneous leishmaniasis (CL). Methods: Forty tissue fragments from early (E-CL) and late CL (L-CL) lesions and from disseminated leishmaniasis (DL) - papules and ulcers - were characterized. The inflamed area per fragment was calculated, and the CD8 and granzyme B expression levels in the infiltrates were quantified by counting positive cells in 15 fields. The localization of CD8 and granzyme B was graded subjectively. Results: Inflammation was higher in L-CL and DL ulcers. CD8 expression was increased in late ulcerated lesions compared to recent lesions. The increase in CD8+ cells also correlated with the duration of the lesion. Papules had a higher frequency of granzyme B+ cells than E-CL lesions, although the frequency was similar to those for late and DL ulcers. CD8+ cells were mostly found in the papillary dermis. Conclusions: CD8+ T and granzyme B+ cells are present in the inflammatory infiltrates of CL and DL and may participate in the immunopathogenesis of Leishmania infection.
