Author(s):
Lima, Luciane Mota ; Santos, Silvane Maria Braga ; Oliveira, Ricardo Riccio ; Cardoso, Luciana Santos ; Oliveira, Sérgio Costa ; Góes, Alfredo Miranda ; Loukas, Alex ; Carvalho Filho, Edgar Marcelino de ; Araujo, Maria Ilma Andrade Santos ; Lima, Luciane Mota ; Santos, Silvane Maria Braga ; Oliveira, Ricardo Riccio ; Cardoso, Luciana Santos ; Oliveira, Sérgio Costa ; Góes, Alfredo Miranda ; Loukas, Alex ; Carvalho Filho, Edgar Marcelino de ; Araujo, Maria Ilma Andrade Santos
Date: 2014
Origin: Oasisbr
Subject(s): Human T cell lymphotropic virus type 1; Schistosoma spp. antigens; Interferon-γ; Interleukin-10
Description
Texto completo: acesso restrito. p. 233-238
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Human T cell lymphotropic virus type 1 (HTLV-1) is the causal agent of HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). While the immune response to HTLV-1 infection is polarized to the Th1-type, chronic helminth infections drive the Th2- and T regulatory-type, and are able to downregulate the inflammatory response in some autoimmune diseases. Objective: To evaluate whether Schistosoma spp. antigens alter the in vitro cytokine response in HTLV-1 infection. Methods: The recombinant Schistosoma antigens Sm29 and ShTSP2 (tetraspanin) and PIII, a fraction of the Schistosoma mansoni adult worm antigen were added to peripheral blood mononuclear cell (PBMC) cultures of HTLV-1-infected individuals and the levels of interferon (IFN)-γ and interleukin (IL)-10 in the supernatants were measured using the ELISA sandwich technique. Results: Compared to the levels of cytokine in nonstimulated cultures, the levels of IFN-γ were reduced in 50, 47 and 50% of patients by the presence of Sm29, ShTsp2 and PIII, respectively. The downregulation of IFN-γ production in the presence of Sm29 antigen was observed mainly in subjects who had lower basal levels of this cytokine. The levels of IL-10, however, increased by the addition of the three antigens in the cultures in 74, 62 and 44% of individuals, respectively. In addition, there was a decrease in the ratio of IFN-γ/IL-10 levels in cultures stimulated with Sm29 and ShTSP2 when compared to nonstimulated ones. Conclusions: The Schistosoma spp. antigens used in this study were able to downmodulate IFN-γ production in vitro in HTLV-1 infection. This may be associated with the increased levels of IL-10 induced by the antigens.
