Autor(es): Martins, L. K. P. ; Nascimento, Iracema Andrade ; Fillmann, Gilberto ; King, R. ; Evangelista, A. J. A. ; Readman, J. W. ; Depledge, M. H. ; Martins, L. K. P. ; Nascimento, Iracema Andrade ; Fillmann, Gilberto ; King, R. ; Evangelista, A. J. A. ; Readman, J. W. ; Depledge, M. H.
Data: 2015
Origem: Oasisbr
Assunto(s): Neutral red retention assay; Lysosomes; Hemocytes; PAHs; Hydrocarbon contaminants; Biomarker
Texto completo: acesso restrito. p.387–396
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Coastal marine environments, especially semienclosed systems such as bays, are under unrelenting stress caused by urban and industrial development. Biomonitoring plays a vital role in strategies to identify, assess, and control stressors. However, due to the magnitude of the challenge there is a demand for new and innovative approaches to provide timely and accessible information to environmental managers and policy makers. The present work aimed to assess hydrocarbon levels in sediments from petroleum-related industrial areas at Todos os Santos Bay (Brazil) and associate them to toxicity-induced responses (neutral red retention (NRR) assay) by the burrowing clam Anomalocardia brasiliana. Surface sediments collected during the dry and rainy seasons were analyzed for aliphatic and aromatic hydrocarbons. At the control site, hydrocarbon levels were low and mainly biogenic. The aliphatic hydrocarbon (“total unresolved complex mixture,” alkanes, and isoprenoids) concentrations indicated a chronic situation with very little “fresh” oil contamination at the oil-related sites. The polycyclic aromatic hydrocarbons indicated sites moderately contaminated by chronic oil and some pyrolytic input. The effects of those contaminants were assessed by the lysosomal NRR assay applied to A. brasiliana hemocytes. Sediment toxicity at the oil-related sites was evidenced by the lowered capacity of the lysosomes to retain the neutral red dye compared to results from the control site. This research indicates that the NRR assay is a useful and efficient screening technique able to discriminate polluted from clean sites.
