Autor(es): Pereira, Dhélio Batista ; Oliveira, Kelly Régia Vieira ; Moreira, Regina ; Oba, Isabel Takano ; Compri, Adriana Parise ; Lemos, Marcilio Figueiredo ; Vasconcelos, Mariana ; Salcedo, Juan Miguel Villalobos
Data: 2016
Origem: Oasisbr
Assunto(s): Hemodiálise; Hemodiálise; Precisão; Precisão; Sorologia – ELISA – PCR; Sorologia – ELISA – PCR
Keywords: CRF – Hemodialysis – HBV – HCV – Accuracy – Serology – ELISA – PCR.
Submitted by ROBERTO PAULO CORREIA DE ARAÚJO (ppgorgsistem@ufba.br) on 2016-09-16T14:39:07Z No. of bitstreams: 1 1_v.8_3.pdf: 160942 bytes, checksum: c45044c8e51c747b424ec866e5b910ed (MD5)
Made available in DSpace on 2016-09-16T14:39:07Z (GMT). No. of bitstreams: 1 1_v.8_3.pdf: 160942 bytes, checksum: c45044c8e51c747b424ec866e5b910ed (MD5) Previous issue date: 2009-09
Abstract: Patients under hemodialysis treatment for chronic renal failure (CRF) are among the groups with the highest prevalence of hepatitis B and C viruses due to frequent blood transfusions and nosocomial transmission. A group of CRF patients living in Porto Velho were tested with serological markers for hepatitis B and C using the ELISA test and molecular biology techniques (PCR). The validity parameters for the serological results were measured based on the PCR results. Of the 128 patients on hemodialysis during the study, 12 (9.4%) were HBsAg positive, 69 (53.9%) were anti-HBc positive, 93 (72.7%) were anti- HBs positive, and 22 (17.2%) were anti-HCV positive. The PCR tests result in 12 (9.4%) HBV-DNA positive and 16 (12.5%) HCV-RNA positive. The accuracy, sensitivity and specificity of ELISA for HBsAg were 90.6%, 50% and 94.8%, and the same parameters were 92.2%, 87.5% and 92.9% for anti-HCV. Based on the results just the negative predictive value for anti-HCV (98,2%) is a reliable test in CRF patients on hemodialysis. Beside that, serial serological and/or molecular tests are the indicated methodology to diagnosis HBV and HCV infection in these patients.
Salvador
