Author(s):
Melo, Stella Maria Barrouin ; Larangeira, Daniela Farias ; Andrade Filho, Fernando Antônio de ; Trigo, Joelma ; Julião, Fred da Silva ; Franke, Carlos Roberto ; Aguiar, Paulo Henrique Palis ; Santos, Washington Luis Conrado dos ; Carvalho, Lain Carlos Pontes de ; Melo, Stella Maria Barrouin ; Larangeira, Daniela Farias ; Andrade Filho, Fernando Antônio de ; Trigo, Joelma ; Julião, Fred da Silva ; Franke, Carlos Roberto ; Aguiar, Paulo Henrique Palis ; Santos, Washington Luis Conrado dos ; Carvalho, Lain Carlos Pontes de
Date: 2012
Origin: Oasisbr
Subject(s): Dog; Leishmaniosis; Parasitological diagnosis; Spleen aspiration; Leishmaniasis
Description
p. 331–339
Submitted by Ana Valéria de Jesus Moura (anavaleria_131@hotmail.com) on 2012-01-26T15:27:13Z No. of bitstreams: 1 S1090023304002412-main.pdf: 220780 bytes, checksum: 9dbbfa0a50049220e24c7231225f2872 (MD5)
Made available in DSpace on 2012-01-26T15:27:13Z (GMT). No. of bitstreams: 1 S1090023304002412-main.pdf: 220780 bytes, checksum: 9dbbfa0a50049220e24c7231225f2872 (MD5) Previous issue date: 2006
The purpose of this study was to evaluate the safety of spleen aspiration as a sampling technique for the parasitological detection by culture and microscopy of Leishmania (chagasi) infantum. Two hundred and nine domiciled dogs from an endemic area for visceral leishmaniasis in Bahia State, Brazil, were studied. Most dogs (87%) were seropositive for anti-L. chagasi antibodies by ELISA. Clinical signs of disease were recorded and the animals monitored during and after spleen puncture in order to detect possible complications associated with the procedure. From a total of 257 splenic punctures in the 209 animals, only three minor events occurred, with no significant consequence for the animals and no association with risk factors. Leishmania was isolated from 149/180 (83%) seropositive dogs, and from 6/26 (23%) seronegative animals. The procedure did not cause adverse side effects or unnecessary suffering and confirmed the diagnosis in a large percentage of dogs. We conclude that spleen aspiration can be considered an effective and safe procedure for the definitive diagnosis of canine visceral leishmaniosis.
