Autor(es): Fraga, I. C. ; Fregoneze, J. B. ; Carvalho, F. L. Q. ; Dantas, K. B. ; Azevedo, C. S. ; Pinho, Cristina Bacellar de ; Silva, E. de Castro e ; Fraga, I. C. ; Fregoneze, J. B. ; Carvalho, F. L. Q. ; Dantas, K. B. ; Azevedo, C. S. ; Pinho, Cristina Bacellar de ; Silva, E. de Castro e
Data: 2012
Origem: Oasisbr
Assunto(s): serotonin reuptake inhibitors; Fos-IR; serotonin; fed state; fasting state
Trabalho completo: acesso restrito, p. 327–334
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In the present study we investigated the effect of acute fluoxetine administration on the expression of c-Fos in the rat brain under two different metabolic conditions: fed and fasting states. Wistar male rats, weighing 220±30g, received i.p. injections of saline solution or fluoxetine (10mg/kg), and were killed 2 h later. The brains were removed after transcardiac perfusion with phosphate-buffered saline followed by paraformaldehyde, and were then processed for immunohistochemistry. Fos-like immunoreactivity was quantified by a computerized system. Fasted animals faced an 18-h suppression of food intake, while fed groups were submitted to an initial 14-h period of fast followed by a 4-h period in which food was freely available. Both in fasting and fed states, fluoxetine-treated animals presented a significant increase in c-Fos expression in hypothalamic areas, limbic structures, circumventricular areas, and in mesencephalic and rhomboencephalic regions, as compared with saline-treated controls. The quantitative comparison of data obtained from fasted and fed animals showed that fasted rats treated with fluoxetine presented a higher c-Fos expression in the ventromedial hypothalamus and the paraventricular nuclei compared with the fed group, while in fluoxetine-treated fed rats c-Fos expression was higher in the arcuate nuclei, medial amygdala, locus coeruleus and dorsal raphe nuclei, as compared with fasted, fluoxetine-treated animals. These data indicate that the metabolic condition of the animals significantly modifies fluoxetine-induced brain c-Fos expression, suggesting that visceral and behavioral fluoxetine effects may be influenced by the metabolic state of the individual.
