Author(s): Machado, Gabriel Capella ; Moris, Daniela Vanessa ; Arantes, Thales Domingos ; Silva, Luciane Regina Franciscone ; Theodoro, Raquel Cordeiro ; Mendes, Rinaldo Poncio [UNESP] ; Vicentini, Adriana Pardini ; Bagagli, Eduardo
Date: 2014
Persistent ID: http://hdl.handle.net/11449/109942
Origin: Oasisbr
Subject(s): Paracoccidioides spp; cryptic species; antigen preparations; immunodiagnosis; gp43
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We aimed to evaluate whether the occurrence of cryptic species of Paracoccidioides brasiliensis, S1, PS2, PS3 and Paracoccidioides lutzii, has implications in the immunodiagnosis of paracoccidioidomycosis (PCM). Small quantities of the antigen gp43 were found in culture filtrates of P. lutzii strains and this molecule appeared to be more variable within P. lutzii because the synonymous-nonsynonymous mutation rate was lower, indicating an evolutionary process different from that of the remaining genotypes. The production of gp43 also varied between isolates belonging to the same species, indicating that speciation events are important, but not sufficient to fully explain the diversity in the production of this antigen. The culture filtrate antigen AgEpm83, which was obtained from a PS3 isolate, showed large quantities of gp43 and reactivity by immunodiffusion assays, similar to the standard antigen (AgB-339) from an S1 isolate. Furthermore, AgEpm83 was capable of serologically differentiating five serum samples from patients from the Botucatu and Jundiaí regions. These patients had confirmed PCM but, were non-reactive to the standard antigen, thus demonstrating an alternative for serological diagnosis in regions in which S1 and PS2 occur. We also emphasise that it is not advisable to use a single antigen preparation to diagnose PCM, a disease that is caused by highly diverse pathogens.
Universidade Estadual Paulista Julio de Mesquita Filho Instituto de Biociencias de Botucatu Departamento de Microbiologia e Imunologia
Universidade Estadual Paulista Julio de Mesquita Filho Faculdade de Medicina de Botucatu Departamento de Doencas Tropicais
Instituto Adolfo Lutz Centro de Imunologia Laboratorio de Imunodiagnostico das Micoses
Universidade Estadual Paulista Julio de Mesquita Filho Instituto de Biociencias de Botucatu Departamento de Microbiologia e Imunologia
Universidade Estadual Paulista Julio de Mesquita Filho Faculdade de Medicina de Botucatu Departamento de Doencas Tropicais
