Author(s): Bustos-Obregon, Eduardo ; Poblete, Daniel ; Catriao, Roberto ; del Sol, Mariano ; Henrique Fernandes, Fabio [UNESP]
Date: 2014
Persistent ID: http://hdl.handle.net/11449/111488
Origin: Oasisbr
Subject(s): Mouse; Melatonin; Arsenic; Sperm; DNA stability
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We evaluated the sperm parameters such as cauda epididymis weight, sperm count, sperm morphology and sperm DNA stability of adult CF-1 male mice treated daily (oral exposure) with the toxic sodium arsenite (As, 7.0 mg/kg/body weight); Melatonin (Me, 10.0 mg/kg/bw), Me (10.0 mg/kg/bw) plus As (7.0 mg/kg/bw) and Negative Control (NaCl 0.9%) to assess acute (8.3 days), chronic (33.2 days) and recovery of testicular damage (66.4 days). Arsenic decreases the number of sperm from chronic treatment (33.2 days) and this effect continued until 66.4 days of treatment. The toxic effect of As also altered the morphology of spermatozoa in all treatment periods when compared to the negative control group. However, Metalonin induced protective effects in periods of 33.2 and 66.4 days of treatment. Additionally, the stability of DNA was significantly affected by arsenic in all periods, but the chronic treatment (33.2 days) in the AsMe revealed increased stability compared to the group treated with arsenic only. Melatonin partially protects sperm toxicity caused by Arsenic, especially during periods of 33.2 and 66.4 days.
Univ La Frontera, Temuco, Chile
Univ Chile, Sch Med, Santiago, Chile
Univ Santo Tomas, Sch Vet, Santiago, Chile
Univ Autonoma Chile, Ctr Invest Ciencias Biomed, Temuco, Chile
Sao Paulo State Univ, Botucatu, SP, Brazil
Sao Paulo State Univ, Botucatu, SP, Brazil
