Autor(es): Rocha, Thalita Leone Alves [UNESP] ; Dias-Junior, Carlos Alan [UNESP] ; Possomato-Vieira, José Sergio [UNESP] ; Gonçalves-Rizzi, Victor Hugo [UNESP] ; Nogueira, Flávia Ribeiro [UNESP] ; Souza, Kátina Meneghetti de [UNESP] ; Braz, Leandro Gobbo [UNESP] ; Braz, Mariana Gobbo [UNESP]
Data: 2015
Identificador Persistente: http://hdl.handle.net/11449/128572
Origem: Oasisbr
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Taking into account that there are controversial antioxidative effects of inhalational anesthetics isoflurane and sevoflurane and absence of comparison of genotoxicity of both anesthetics in animal model, the aim of this study was to compare DNA damage and antioxidant status in Wistar rats exposed to a single time to isoflurane or sevoflurane. The alkaline single-cell gel electrophoresis assay (comet assay) was performed in order to evaluate DNA damage in whole blood cells of control animals (unexposed; n = 6) and those exposed to 2% isoflurane (n = 6) or 4% sevoflurane (n = 6) for 120 min. Plasma antioxidant status was determined by 3( 4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. There was no statistically significant difference between isoflurane and sevoflurane groups regarding hemodynamic and temperature variables (P > 0.05). Sevoflurane significantly increased DNA damage compared to unexposed animals (P = 0.02). In addition, Wistar rats anesthetized with isoflurane showed higher antioxidative status (MTT) than control group (P = 0.019). There were no significant differences in DNA damage or antioxidant status between isoflurane and sevoflurane groups (P > 0.05). In conclusion, our findings suggest that, in contrast to sevoflurane exposure, isoflurane increases systemic antioxidative status, protecting cells from DNA damage in rats.
Universidade Estadual Paulista, Departamento de Farmacologia, Instituto de Biociências de Botucatu
Universidade Estadual Paulista, Departamento de Anestesiologia, Faculdade de Medicina de Botucatu
