Author(s): Monteiro, Nilson Kobori [UNESP] ; Aranda-Selverio, Gabriel [UNESP] ; Exposti, Diego Tadeu Degli [UNESP] ; Silva, Maria de Lourdes Corradi da [UNESP] ; Lemos, Eliana Gertrudes Macedo [UNESP] ; Campanharo, João Carlos [UNESP] ; Silveira, Joana Léa Meira
Date: 2014
Persistent ID: http://hdl.handle.net/11449/130390
Origin: Oasisbr
Subject(s): Acid exopolysaccharide; Rhizobium tropici; Mesorlazobium sp.
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
The exopolysaccharides with characteristics of gel produced by Rhizobium tropici (EPS RT) and Mesorhizobium sp (EPS MR) are acidic heteropolysaccharide composed mainly of glucose and galactose in a molar ratio of 4:1 and 5:1 respectively, with traces of mannose (~ 1%). Chemical analysis showed the presence of uronic acid, pyruvate and acetyl-substituents in the structures of both polymers. Experiments of gel permeation chromatography and polyacrylamide gel electrophoresis showed that EPS RT and EPS MR are homogeneous molecules with low grade of polydispersity. The EPS were characterized using spectroscopic techniques of FT-IR, ¹H and 13C-NMR.
Univ Estadual Paulista, Dept Engn & Tecnol Alimentos, Inst Biociencias Letras & Ciencias Exatas, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
Univ Estadual Paulista, Fac Ciencias & Tecnol, Dept Fis Quim & Biol, BR-19060900 Presidente Prudente, SP, Brazil
Univ Estadual Paulista, Dept Tecnol, Fac Ciencias Agr & Vet, BR-14884900 Jaboticabal, SP, Brazil
Universidade Federal do Paraná (UFPR), Dept Bioquim & Biol Mol, BR-81531990 Curitiba, Parana, Brazil
Univ Estadual Paulista, Dept Engn & Tecnol Alimentos, Inst Biociencias Letras & Ciencias Exatas, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
Univ Estadual Paulista, Fac Ciencias & Tecnol, Dept Fis Quim & Biol, BR-19060900 Presidente Prudente, SP, Brazil
Univ Estadual Paulista, Dept Tecnol, Fac Ciencias Agr & Vet, BR-14884900 Jaboticabal, SP, Brazil
