Author(s): Thyssen, Patrícia Jaqueline ; Nassau, Mariana Prado ; Nitsche, Maria José Trevizani [UNESP] ; Leite, Domingos da Silva
Date: 2016
Persistent ID: http://hdl.handle.net/11449/136911
Origin: Oasisbr
Subject(s): Diptera; Wounds; Treatment; Maggots; Biotherapy
Made available in DSpace on 2016-04-01T18:43:15Z (GMT). No. of bitstreams: 0 Previous issue date: 2013. Added 1 bitstream(s) on 2016-04-01T18:49:15Z : No. of bitstreams: 1 ISSN2141-9477-2013-04-10-405-409.pdf: 238922 bytes, checksum: 2495ae3a9b45b31f14b3392bec437af5 (MD5)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
The larval therapy involves the intentional application of sterilized and alive dipterans larvae on nonhealing wounds with the purpose of cleaning out the necrotic tissue to facilitate the healing process. This study aimed to evaluate the sterilization process and survival of Chrysomya megacephala (Fabricius), Chrysomya putoria (Wiedemann) and Cochliomyia macellaria (Fabricius) (Diptera: Calliphoridae) larvae, after treatment with sodium hypochlorite (NaClO). The experimental groups for each species were: control group (CG), in which it was used water; 0.5% sodium hypochlorite group (0.5% SHG); and 1% SHG. For testing the time for each treatment, the eggs were washed for one or three minutes in the described solution. After, aliquots from the "rinsing water" were inoculated in Plate Count Agar (PCA) and blood agar (BA), with incubation at 37ºC and readings performed after 24 and 48 hours for both. For all species, there were no bacterial growth on PCA and BA regarding to both treatments, 0.5% SHG and 1.0% SHG. The survival rate and intervals of immersion were similar among the CG and the different concentrations of NaClO for all species. In conclusion, sterilization of blowflies' eggs made by NaClO is an efficient method to obtain the sterilized larvae for larval therapy.
Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Faculdade de Medicina de Botucatu (FMB), Departamento de Enfermagem, Botucatu, SP, Brasil
Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Faculdade de Medicina de Botucatu (FMB), Departamento de Enfermagem, Botucatu, SP, Brasil
FAPESP: 2012/06033-5
