Autor(es): Araújo, Maria José Abigail Mendes [UNESP] ; Búfalo, Michelle Cristiane [UNESP] ; Conti, Bruno José [UNESP] ; Fernandes Jr., Ary [UNESP] ; Trusheva, Boryana ; Bankova, Vassya ; Sforcin, José Maurício [UNESP]
Data: 2016
Identificador Persistente: http://hdl.handle.net/11449/137218
Origem: Oasisbr
Assunto(s): Chemical composition; Cytokine; Geopropolis; Human laryngeal epidermoid-2 cells; Pharmacological activities
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Aim: Geopropolis is produced by stingless bees from resinous materials of plants, adding salivary secretions, wax, mud or clay, and has been used in folk medicine for the treatment of respiratory diseases and dermatoses. Therefore, it is important to study its antibacterial, antitumor and immunomodulatory properties are important to confirm the ethnopharmacological applications. This work aimed to evaluate its chemical composition, and its antimicrobial, anti-tumoral and immunomodulatory activities. Materials and Methods: Geopropolis composition was investigated using GC-MS analysis. Antibacterial tests were performed to determine the minimum inhibitory concentration. HEp-2 cells viability was determined by the reduction of MTT, and cytokine production by human monocytes was determined by ELISA. Results: The major constituents of geopropolis compounds were carbohydrates and their derivatives, triterpenes, anacardic acid, alkylresorcinols, and sugar alcohols. Geopropolis alone showed no antibacterial activity against Staphylococcus aureus and Escherichia coli, but its combination with chloramphenicol exerted a greater action against S. aureus than chloramphenicol alone. Geopropolis exhibited a cytostatic action toward human laryngeal epidermoid carcinoma cells and stimulated tumor necrosis factor alpha and interleukin-10 production by human monocytes, showing an activator profile for human monocytes. Conclusion: The synergistic effect of geopropolis and chloramphenicol deserves further investigation due to bacterial resistance to antibiotics. Geopropolis also displayed antitumoral and immunomodulatory activity, and its biological properties may be due to triterpenes - one of its major chemical constituents.
Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Instituto de Biociências de Botucatu (IBB), Departamento de Microbiologia e Imunologia, Botucatu, SP, Brasil
Bulgarian Academy of Sciences, Institute of Organic Chemistry with Centre of Phytochemistry, Sofia, Bulgaria
Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Instituto de Biociências de Botucatu (IBB), Departamento de Microbiologia e Imunologia, Botucatu, SP, Brasil
FAPESP: 2010/50064-7
FAPESP: 2010/01028-8
