Author(s): Gomes Junior, Antonio Luiz ; Correia Jardim Paz, Marcia Fernanda ; Soares da Silva, Lais Iasmin ; Carvalho, Simone da Costa e Silva [UNESP] ; Pinho Sobral, Andre Luiz ; Machado, Katia da Conceicao ; Pinheiro Ferreira, Paulo Michel ; Satyal, Prabodh ; Freitas, Rivelilson Mendes de ; Carvalho Melo Cavalcante, Ana Amleia de
Date: 2018
Persistent ID: http://hdl.handle.net/11449/158581
Origin: Oasisbr
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundacao deAmparo e Pesquisa do Estado do Piaui (FAPEPI)
The aim of this study was to evaluate the oxidative parameters of erythrocytes and genotoxicity in leukocytes of patients with breast cancer. Oxidative parameters were detected by spectrophotometry and genotoxic damage by single cell gel electrophoresis. Twenty-eight women with breast cancer were monitored before chemotherapy and after the second and fourth cycles of therapy with cyclophosphamide and doxorubicin. After the fourth cycle, increases (P < 0.05) in the reactive substances to thiobarbituric acid levels, nitrite content, and superoxide dismutase activity and high rates of DNA damage in leukocytes were observed when compared with healthy women group and baseline levels. Similarly, after the second cycle, the same parameters were increased (P < 0.05) when compared with baseline levels. Increase in catalase activity was detected only after the fourth cycle and reduced glutathione levels and glutathione peroxidase activity were decreased in all cycles when compared with healthy women, as well as after the second and fourth chemotherapy cycles compared to baseline (P < 0.05). Patients with breast cancer presented an indicative of oxidative stress before, during, and after chemotherapy, as well as increased genotoxic damage in all stages of treatment, demonstrating the clinical applicability of this investigation.
Univ Fed Piaui, Lab Genet Tox, Postgrad Program Pharmaceut Sci, BR-64049550 Teresina, PI, Brazil
State Univ Sao Paulo, Postgrad Program Genet, BR-14049900 Ribeirao Preto, SP, Brazil
Univ Fed Piaui, Dept Physiol & Biophys, Postgrad Program Pharmaceut Sci, Lab Expt Cancerol, BR-64049550 Teresina, PI, Brazil
Univ Alabama, Dept Chem, Huntsville, AL 35899 USA
Univ Fed Piaui, Lab Res Expt Neurochem, Postgrad Program Pharmaceut Sci, BR-64049550 Teresina, PI, Brazil
State Univ Sao Paulo, Postgrad Program Genet, BR-14049900 Ribeirao Preto, SP, Brazil
