Author(s): Campos-Verdes, Larysse Maira ; Silva-Sampaio, Joao Paulo da ; Costa-Silva, Danylo Rafhael ; Oliveira, Victor Alves de ; Conde Junior, Airton Mendes ; Silva, Vladimir Costa ; Alencar, Airlane Pereira [UNESP] ; Campelo, Viriato ; Lopes-Costa, Pedro Vitor ; Gebrim, Luiz Henrique ; Silva, Benedito Borges da
Date: 2018
Persistent ID: http://hdl.handle.net/11449/160157
Origin: Oasisbr
Subject(s): Breast cancer; Calcium; CaSR polymorphism; Menopausal status; qPCR
Made available in DSpace on 2018-11-26T15:47:41Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-03-01
Breast cancer is a disease of unknown etiology, whose major risk factors are genetic alterations. Polymorphism of the calcium-sensing receptor (CaSR) has been a focus of some recent studies, due to a probable association with breast cancer risk and tumor aggressiveness. A relationship between polymorphic rs17251221 variant of the CaSR gene, and allele G (considered a gain-of-function mutation) and breast cancer risk has been stressed, despite the paucity of studies found in the literature. The present study involved 137 women (69 women with breast cancer-case; and 68 controls without breast cancer) who had 3 ml of peripheral blood drawn for DNA study. Genomic DNA was extracted from leukocytes by genotyping technique with real-time polymerase chain reaction. The AG genotype (rs17251221) was present in 13 women (18.84%) from the case group and in 8 (11.76%) women from the control group (p = 0.3434), while the GG genotype (rs17251221) did not occur in any group. In contrast, no statistically significant difference was observed between the AG genotype of variant rs17251221 in premenopausal case and control women (p = 0.71). There was also no statistically significant difference between postmenopausal case and control patients (p = 0.6851). In the current study, CaSR gene polymorphism of SNP variant rs17251221 did not show any statistically significant association with breast cancer, in both premenopausal and postmenopausal women.
Univ Fed Piaui, Postgaduate Program Hlth & Sci, BR-64049550 Teresina, Brazil
Univ Fed Piaui, Postgrad Program Northeast Network Biotechnol REN, BR-64049550 Teresina, Brazil
Univ Fed Piaui, Natan Portella Hosp, Dept Mol Biol, BR-64001450 Teresina, Brazil
State Univ Sao Paulo, Dept Stat, BR-03178200 Sao Paulo, Brazil
Univ Fed Sao Paulo, Dept Mastol, BR-04023900 Sao Paulo, Brazil
State Univ Sao Paulo, Dept Stat, BR-03178200 Sao Paulo, Brazil
