Author(s): Steffler, Lizandra Makowski ; Dolabella, Silvio Santana ; Martins Ribolla, Paulo Eduardo [UNESP] ; Dreyer, Carine Spenassatto [UNESP] ; Araujo, Edilson Divino ; Oliveira, Rosane Gomes ; Silva Martins, Walter Fabricio ; La Corte, Roseli
Date: 2018
Persistent ID: http://hdl.handle.net/11449/162001
Origin: Oasisbr
Subject(s): ISSR-PCR; Single nucleotide polymorphism; Entomological surveillance; Vector control; Zika; Dengue; Chikungunya; Arboviruses
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FAPITEC
Background: The study of the genetic structure of Aedes aegypti is essential to understanding their population dynamics as well as for the analysis of factors responsible for their resistance and ecological adaptation. The use of molecular markers in identifying differences amongst populations of Ae. aegypti in different geographical areas as well as the temporal variation of the vector populations has contributed to the improvement of vector control strategies. The present study aims to determine the genetic variability of Ae. aegypti populations in a small geographical area (state of Sergipe, Northeastern Brazil) by means of inter-simple sequence repeat (ISSR) and single nucleotide polymorphism (SNP) molecular markers. Results: ISSR markers revealed a more heterogeneous pattern of genetic diversity among the populations with an expected heterozygosity (HE) ranging from 0.261 +/- 0.03 to 0.120 +/- 0.032, while a similar trend was detected through SNPs across populations with an HE between 0.375 +/- 0.054 and 0.269 +/- 0.042. The population's genetic differentiation assessed with ISSR and SNP markers indicated a very low structuring among the populations with the highest diversity observed within the populations 72 % (ISSR) and 92 % (SNP). Clustering analysis also suggested little variation among populations: the seven populations were grouped into only three ISSR clusters and a single panmictic group based on SNP markers. The present study identified a close relationship between the populations, which probably results mainly from passive gene flow between mosquitoes from distinct geographic regions, influenced by humans commuting along roads. Conclusions: There was an intense migration of mosquitos across municipalities, leading to a potential increase in risk of arbovirus and insecticide resistance associated-alleles spreading between mosquito populations.
Univ Fed Sergipe, Programa Posgrad Biol Parasitaria, Ave Mal Rondon S-N, BR-49100000 Sao Cristovao, Sergipe, Brazil
Univ Fed Sergipe, Dept Morfol, Ave Mal Rondon S-N, BR-49100000 Sao Cristovao, Sergipe, Brazil
Univ Estadual Julio de Mesquita Filho, Dept Parasitol, Rubiao Jr,CP 510, BR-18618900 Botucatu, SP, Brazil
Univ Fed Sergipe, Dept Biol, Ave Mal Rondon S-N, BR-49100000 Sao Cristovao, Sergipe, Brazil
Univ Fed Sergipe, Programa Posgrad Biotecnol, Rede Nordeste Biotecnol, Ave Mal Rondon S-N, BR-49100000 Sao Cristovao, Sergipe, Brazil
Univ Estadual Paraiba, Dept Biol CCBS, R Baraunas 351, BR-58429500 Campina Grande, Paraiba, Brazil
Univ Estadual Julio de Mesquita Filho, Dept Parasitol, Rubiao Jr,CP 510, BR-18618900 Botucatu, SP, Brazil
