Autor(es): Lorenzoni, Andre S. G. ; Dantas, Giordanni C. [UNESP] ; Bergsma, Tessa ; Ferreira, Henrique [UNESP] ; Scheffers, Dirk-Jan
Data: 2018
Identificador Persistente: http://hdl.handle.net/11449/163049
Origem: Oasisbr
Assunto(s): Xanthomonas citri; MinC; FtsZ; ZapA; ParB; peptidoglycan; citrus canker
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Netherlands Organisation for Scientific research (NWO)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
NWO Vidi
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Xanthomonas citri (Xac) is the causal agent of citrus canker, a disease that affects citrus crops and causes economic impact worldwide. To further characterize cell division in this plant pathogen, we investigated the role of the protein MinC in cell division, chromosome segregation, and peptidoglycan incorporation by deleting the gene minC using allele exchange. Xac with minC deleted exhibited the classic Amin phenotype observed in other bacteria deleted for min components: minicells and short filamentation. In addition we noticed the formation of branches, which is similar to what was previously described for Escherichia coli deleted for either min or for several low molecular weight penicillin-binding proteins (PBPs). The branching phenotype was medium dependent and probably linked to gluconeogenic growth. We complemented the minC gene by integrating gfp-minC into the amy locus. Xac complemented strains displayed a wild-type phenotype. In addition, GFP-MinC oscillated from pole to pole, similar to MinCD oscillations observed in E coli and more recently in Synechococcus elongatus. Further investigation of the branching phenotype revealed that in branching cells nucleoid organization, divisome formation and peptidoglycan incorporation were disrupted.
Univ Groningen, Groningen Biomol Sci & Biotechnol Inst, Dept Mol Microbiol, Groningen, Netherlands
Univ Estadual Paulista, Inst Biociencias, Dept Bioquim & Microbiol, Rio Claro, Brazil
Univ Estadual Paulista, Inst Biociencias, Dept Bioquim & Microbiol, Rio Claro, Brazil
Netherlands Organisation for Scientific research (NWO): 729.004.005
FAPESP: 2013/50367-8
FAPESP: 2013/14013-7
NWO Vidi: 864.09.010
CNPq: 246986/2013-1
