Author(s): Salgado-Neto, Geraldo ; Braz Vaz, Marcos Andre ; Carus Guedes, Jerson Vanderlei ; Briao Muniz, Marlove Fatima ; Blume, Elena ; Wilcken, Carlos Frederico [UNESP] ; Castro e Castro, Barbara Monteiro de ; Plata-Rueda, Angelica ; Zanuncio, Jose Cola
Date: 2018
Persistent ID: http://hdl.handle.net/11449/163798
Origin: Oasisbr
Made available in DSpace on 2018-11-26T17:45:01Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-02-05
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
Programa Cooperativo sobre Protecao Florestal (PROTEF) do Instituto de Pesquisas e Estudos Florestais (IPEF)
The dispersion of pathogenic microorganisms consists of the transport of pathogens from their source to inoculate a new host. Agricultural and economic importance of the Soybean root rot justifies studying this disease, especially the role of insects as dispersers. The aim of this study was to evaluate the role of the ladybird beetle, Cycloneda sanguinea Linnaeus (Coleoptera: Coccinellidae) in the dispersion of pathogens that cause Soybean root rot. Three pathogen species, Macrophomina phaseolina (Tassi) (Sphaeropsidales: Botryosphaeriaceae), Fusarium incarnatum-equiseti species complex (FIESC), and F. commune (Skovgaard) O'Donnell & Nirenberg were isolated from the midgut of ladybird beetles and cultured. Macrophomina phaseolina was identified by morphology while for the other two species, DNA was sequenced. The DNA extracted was amplified in the Internal Transcriber Spacer (ITS) region, sequenced and compared to voucher sequences deposited in the GenBank. Sequences of nucleotide ITS1-5.8 S were identified in the regions of rDNA-ITS4 ribosomal DNA. This is the first report of Macrophomina phaseolina (Tassi) (Sphaeropsidales: Botryosphaeriaceae), Fusarium incarnatum-equiseti species complex (FIESC), and F. commune (Skovgaard) O'Donnell & Nirenberg, being dispersed by C. sanguinea in Brazilian soybean fields.
Univ Fed Santa Maria, Dept Def Fitossanit, BR-97105900 Santa Maria, RS, Brazil
Univ Fed Santa Maria, Dept Estat, BR-97105900 Santa Maria, RS, Brazil
Univ Fed Vicosa, Dept Entomol, BR-38810000 Rio Paranaiba, Brazil
Univ Estadual Paulista, Dept Protecao Plantas, Escola Ciencias Agron, BR-18603970 Botucatu, SP, Brazil
Univ Fed Vicosa, Dept Fitotecnia, BR-36570900 Vicosa, MG, Brazil
Univ Fed Vicosa, Dept Entomol BIOAGRO, BR-36570900 Vicosa, MG, Brazil
Univ Estadual Paulista, Dept Protecao Plantas, Escola Ciencias Agron, BR-18603970 Botucatu, SP, Brazil
