Autor(es): Leao, Evelynne Urzedo ; Almeida Spadotti, David Marques de ; Rocha, Kelly Cristina G. ; Lima, Elison Fabricio B. ; Tavella, Luciana ; Turina, Massimo ; Krause-Sakate, Renate [UNESP]
Data: 2018
Identificador Persistente: http://hdl.handle.net/11449/164336
Origem: Oasisbr
Assunto(s): molecular identification; real-time PCR; tospovirus vector
Made available in DSpace on 2018-11-26T17:52:11Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-01-01. Added 1 bitstream(s) on 2019-10-09T18:27:33Z : No. of bitstreams: 1 S1516-89132017000100454.pdf: 633133 bytes, checksum: 863802737accb4c798370ebe32c2a764 (MD5)
Identification of Thysanoptera is based mainly on external morphology examination that can be time-consuming and difficult for non taxonomic experts. In this work, we propose a rapid and efficient molecular method to identify Frankliniella schultzei, an important and widespread pest thrips vector of tospoviruses in South America countries. Species-specific primers designed in the mitochondrial cytochrome oxidase I gene (mtCOI) were optimized for detection by conventional PCR and real-time PCR. The primers were tested on immature and adult thrips collected from crops and weeds found in Sao Paulo State. All samples collected were identified as F. schultzei, indicating the high prevalence of this species as vector of tospoviruses in Brazilian fields.
Univ Estadual Sao Paulo, Fac Ciencias Agron, Dept Protecao Vegetal Botucatu, Sao Paulo, Brazil
Univ Fed Piaui, UFPI, Dept Biol, Ctr Ciencias Nat, Teresina, Piaui, Brazil
Univ Turin, Scuola Agr & Med Vet, Dipartimento Sci Agr Forestali & Alimentari, ULF Entomol Gen & Appl Grugliasco, Turin, Italy
Ist Protez Sostenibile Piante Turin, Consiglio Nazl Ric, Curitiba, Parana, Brazil
Univ Estadual Paulista, Dept Protecao Vegetal, Botucatu, SP, Brazil
Univ Estadual Paulista, Dept Protecao Vegetal, Botucatu, SP, Brazil
