Autor(es): Savietto, Abigail [UNESP] ; Polaquini, Carlos Roberto [UNESP] ; Kopacz, Malgorzata ; Scheffers, Dirk-Jan ; Marques, Beatriz Carvalho [UNESP] ; Regasini, Luis Octavio [UNESP] ; Ferreira, Henrique [UNESP]
Data: 2018
Identificador Persistente: http://hdl.handle.net/11449/164351
Origem: Oasisbr
Assunto(s): Citrus canker; Gallic acid; Cell division; Membrane disruption
Made available in DSpace on 2018-11-26T17:52:14Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-08-01
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
bilateral research program Biobased Economy from the Netherlands Organization for Scientific research
INCT Citros
Asiatic citrus canker (ACC) is an incurable disease of citrus plants caused by the Gram-negative bacterium Xanthomonas citri subsp. citri (X. citri). It affects all the commercially important citrus varieties in the major orange producing areas around the world. Control of the pathogen requires recurrent sprays of copper formulations that accumulate in soil and water reservoirs. Here, we describe the improvement of the alkyl gallates, which are potent anti-X. citri compounds, intended to be used as alternatives to copper in the control of ACC. Acetylation of alkyl gallates increased their lipophilicity, which resulted in potentiation of the antibacterial activity. X. citri exposed to the acetylated compounds exhibited increased cell length that is consistent with the disruption of the cell division apparatus. Finally, we show that inhibition of cell division is an indirect effect that seemed to be caused by membrane permeabilization, which is apparently the primary target of the acetylated alkyl gallates.
Univ Estadual Paulista, Inst Biociencias, Dept Bioquim & Microbiol, Ave 24A,1515, BR-13506900 Rio Claro, SP, Brazil
Univ Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, Dept Quim & Ciencias Ambientais, Rua Cristovao Colombo 2265, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil
Univ Groningen, Groningen Biomol Sci & Biotechnol Inst, Dept Mol Microbiol, Nijenborgh 7, NL-9747 AG Groningen, Netherlands
Univ Estadual Paulista, Inst Biociencias, Dept Bioquim & Microbiol, Ave 24A,1515, BR-13506900 Rio Claro, SP, Brazil
Univ Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, Dept Quim & Ciencias Ambientais, Rua Cristovao Colombo 2265, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil
FAPESP: 2014/114025
bilateral research program Biobased Economy from the Netherlands Organization for Scientific research: NWO 729.004.005
FAPESP: FAPESP 2013/50367-8
INCT Citros: FAPESP 2014/50880-0
INCT Citros: CNPq 465440/2014-2
